Cloning by nuclear transfer using mammalian somatic cells has enormous potential
application. However, somatic cloning has been inefficient in all species in which live clones …

Fertile offspring from somatic cell nuclear transfer (SCNT) is the goal of most cloning
laboratories. For this process to be successful, a number of events must occur correctly. First …

Puromycin N-acetyl transferase gene (pac), of which the gene product catalyzes antibiotic
puromycin (an effective inhibitor of protein synthesis), has been widely used as a dominant …

Background Somatic cell nuclear transfer (SCNT) using genetically engineered donor cells
is currently the most widely used strategy to generate tailored pig models for biomedical …

This study was conducted to improve a porcine somatic cell nuclear transfer (SCNT)
technique by optimizing donor cell and recipient oocyte preparations. Adult and fetal …

Nutrigenomics in dairy cows is a relatively new area of research. It is defined as the study of
the genomewide influences of nutrition altering the expression of genes. Dietary compounds …

The efficiency of nuclear transfer (NT) using two primary cultures of fetal fibroblasts (FF1 and
FF2) was compared vs. the same cultures transfected with an expression vector in which the …

In vitro transfection of cultured cells combined with nuclear transfer currently is the most
effective procedure to produce transgenic livestock. In the present study, bovine primary fetal …

To assess sources of variation in nuclear transfer efficiency, bovine fetal fibroblasts (BFF),
harvested from six Jersey fetuses, were cultured under various conditions. After transfection …

This study examined bovine cloning strategies that may be used for gene targeting in
animals of known phenotypic traits. Fibroblast cells derived from an adult and a fetus of the …