Highlights•De novo designed catalysts still are barely matching catalytic efficiency of small
molecules.•Best design strategies focus on the active site functional tuning and protein …

Diagnostic assays that rely on molecular interactions have come a long way; from initial
reversible detection systems towards irreversible reaction indicator-based methods. More …

MicroRNA-21 (miR-21) is known to act as an important biomarker for cancer, in that its up-
regulation is closely related to several types of malignant tumor. Sensitive and accurate …

Design of a new catalytic function in proteins, apart from its inherent practical value, is
important for fundamental understanding of enzymatic activity. Using a computationally …

Engineered cytochrome P450 monooxygenase variants are reported as highly active and
selective catalysts for the bioorthogonal uncaging of propargylic and benzylic ether …

We present a quartz crystal microbalance (QCM) immunosensor for highly sensitive
detection of prostate-specific antigen (PSA) in a human serum immunoassay. In particular, in …

Hydrogen peroxide (H2O2) detection is of high importance as it is a versatile (bio) marker
whose detection can indicate the presence of explosives, enzyme activity and cell signalling …

Naked‐eye detection of an analyte at an ultralow concentration in a complex mixture is the
ultimate goal of an analytical chemist. Over the years, with a wide range of sophisticated …

Signal transduction and signal amplification are both important mechanisms used within
biological signalling pathways. Inspired by this process, we have developed a signal …

Two single-molecule, self-immolative fluoride probes, namely tert-butyldimethylsilyl-
protected 2-and 4-difluoromethylphenol, are described. Compared to similar systems …