Optogenetics holds the promise of controlling biological processes with superb temporal
and spatial resolution at minimal perturbation. Although many of the light-reactive proteins …

Harnessing the potential of optogenetics in biology requires methodologies from different
disciplines ranging from biology, to mechatronics engineering, to control engineering. Light …

The design and implementation of synthetic circuits that operate robustly in the cellular
context is fundamental for the advancement of synthetic biology. However, their practical …

The ability to perform sophisticated, high-throughput optogenetic experiments has been
greatly enhanced by recent open-source illumination devices that allow independent …

Bioluminescent technologies are amongst the most commonly used tools for quantifying and
visualising biological processes, and novel functions are being engineered for their …

Abstract Motivated by Maxwells-Demon, we propose and solve a cellular control problem in
which the exploitation of stochastic noise can break symmetry between two cells and allow …

This thesis explores, implements, and examines the methods to improve the efficiency of
model calibration experiments for synthetic biological circuits in three aspects: experimental …