The intracellular environment represents an extremely crowded milieu, with a limited amount
of free water and an almost complete lack of unoccupied space. Obviously, slightly salted …

Biological macromolecules evolve and function within intracellular environments that are
crowded with other macromolecules. Crowding results in surprisingly large quantitative …

Detailed knowledge of the rates, equilibria, and mechanism of biochemical reactions has
traditionally been acquired through experiments conducted on solutions containing low …

To investigate the consequences of macromolecular crowding on the behavior of a globular
protein, we performed a combined experimental and computational study on the 148 …

The concept of excluded volume and possible effects of excluded volume on the reactivity of
macromolecules in highly volume-occupied or 'crowded'media are introduced and briefly …

Background The environment inside cells in which proteins fold and function are quite
different from that of the dilute buffer solutions often used during in vitro experiments. The …

Macromolecular crowding is expected to have a significant effect on protein aggregation. In
the present study we analyzed the effect of macromolecular crowding on fibrillation of four …

Background Cellular interior is known to be densely crowded due to the presence of soluble
and insoluble macromolecules, which altogether occupy~ 40% of the total cellular volume …

Here we show that increased amount of secondary structure is acquired in the folded states
of two structurally-different proteins (α-helical VlsE and α/β flavodoxin) in the presence of …

The folding of multisubunit proteins is of tremendous biological significance since the large
majority of proteins exist as protein–protein complexes. Extensive experimental and …