It's not just a phase: function and characteristics of RNA-binding proteins in phase separation

HJ Wiedner, J Giudice - Nature structural & molecular biology, 2021 - nature.com
Biomolecular condensates that form via phase separation are increasingly regarded as
coordinators of cellular reactions that regulate a wide variety of biological phenomena …

Phase separation in viral infections

H Li, C Ernst, M Kolonko-Adamska… - Trends in …, 2022 - cell.com
Viruses rely on the reprogramming of cellular processes to enable efficient viral replication;
this often requires subcompartmentalization within the host cell. Liquid–liquid phase …

Aging can transform single-component protein condensates into multiphase architectures

A Garaizar, JR Espinosa, JA Joseph… - Proceedings of the …, 2022 - National Acad Sciences
Phase-separated biomolecular condensates that contain multiple coexisting phases are
widespread in vitro and in cells. Multiphase condensates emerge readily within …

Let's phase it: viruses are master architects of biomolecular condensates

SM Sagan, SC Weber - Trends in Biochemical sciences, 2023 - cell.com
Viruses compartmentalize their replication and assembly machinery to both evade detection
and concentrate the viral proteins and nucleic acids necessary for genome replication and …

Structural analysis of SARS-CoV-2 genome and predictions of the human interactome

A Vandelli, M Monti, E Milanetti, A Armaos… - Nucleic acids …, 2020 - academic.oup.com
Specific elements of viral genomes regulate interactions within host cells. Here, we
calculated the secondary structure content of> 2000 coronaviruses and computed> 100 000 …

RNA: The Unsuspected Conductor in the Orchestra of Macromolecular Crowding

E Zacco, L Broglia, M Kurihara, M Monti… - Chemical …, 2024 - ACS Publications
This comprehensive Review delves into the chemical principles governing RNA-mediated
crowding events, commonly referred to as granules or biological condensates. We explore …

Protein structural transitions critically transform the network connectivity and viscoelasticity of RNA-binding protein condensates but RNA can prevent it

AR Tejedor, I Sanchez-Burgos… - Nature …, 2022 - nature.com
Biomolecular condensates, some of which are liquid-like during health, can age over time
becoming gel-like pathological systems. One potential source of loss of liquid-like properties …

[HTML][HTML] Liquid-liquid phase separation of nucleocapsid proteins during SARS-CoV-2 and HIV-1 replication

BA Chau, V Chen, AW Cochrane, LJ Parent… - Cell Reports, 2023 - cell.com
The leap of retroviruses and coronaviruses from animal hosts to humans has led to two
ongoing pandemics and tens of millions of deaths worldwide. Retrovirus and coronavirus …

Computational resources for identifying and describing proteins driving liquid–liquid phase separation

R Pancsa, W Vranken, B Mészáros - Briefings in Bioinformatics, 2021 - academic.oup.com
One of the most intriguing fields emerging in current molecular biology is the study of
membraneless organelles formed via liquid–liquid phase separation (LLPS). These …

O-Linked-N-Acetylglucosaminylation of the RNA-Binding Protein EWS N-Terminal Low Complexity Region Reduces Phase Separation and Enhances Condensate …

ML Nosella, M Tereshchenko, I Pritisanac… - Journal of the …, 2021 - ACS Publications
Many membraneless organelles are thought to be biomolecular condensates formed by
phase separation of proteins and other biopolymers. Post-translational modifications (PTMs) …