Gene therapy of genetically determined diseases, including some pathologies of the
respiratory system, requires an efficient method for transgene delivery. Recombinant adeno …

High expression level and long-term expression stability are required for therapeutic protein
production in mammalian cells. Three commonly used promoters from the simian virus 40 …

The properties of constitutive promoters within adeno-associated viral (AAV) vectors have
not yet been fully characterized. In this study, AAV vectors, in which enhanced GFP …

The unintended consequences of gene targeting in mouse models have not been
thoroughly studied and a more systematic analysis is needed to understand the frequency …

The human cytomegalovirus promoter (hCMV) is susceptible to gene silencing in CHO cells,
most likely due to epigenetic events, such as DNA methylation and histone modifications …

Background The production of recombinant proteins in mammalian cell lines is one of the
most important areas in biopharmaceutical industry. Viral transcriptional promoters are …

Single-domain antibodies have emerged as highly versatile nanoprobes for advanced
cellular imaging. For real-time visualization of endogenous antigens, fluorescently labelled …

Introduction Human induced pluripotent stem cells (iPSCs), with their ability to generate
human neural cells (astrocytes and neurons) from patients, hold great promise for …

In utero electroporation (IUE) is a simple and rapid approach to in vivo investigate
exogenous gene function in mouse brain, and intensive studies using IUE have greatly …

Intervertebral disc degeneration is the main contributor to low back pain, now the leading
cause of disability worldwide. Gene transfer, either in a therapeutic attempt or in basic …