Evolutionary changes in organismal traits may occur either gradually or suddenly. However,
until recently, there has been little direct information about how phenotypic changes are …

Understanding the mechanisms of evolution requires information on the rate of appearance
of new mutations and their effects at the molecular and phenotypic levels. Although …

Mutations generate sequence diversity and provide a substrate for selection. The rate of de
novo mutations is therefore of major importance to evolution. Here we conduct a study of …

The RNA-guided DNA endonuclease Cas9 is a powerful tool for genome editing. Little is
known about the kinetics and fidelity of the double-strand break (DSB) repair process that …

Understanding why some species have more genetic diversity than others is central to the
study of ecology and evolution, and carries potentially important implications for …

CRISPR/Cas9 gene drive (CGD) promises to be a highly adaptable approach for spreading
genetically engineered alleles throughout a species, even if those alleles impair …

Mutation is the ultimate source of genetic variation. The most direct and unbiased method of
studying spontaneous mutations is via mutation accumulation (MA) lines. Until recently, MA …

To take complete advantage of information on within-species polymorphism and divergence
from close relatives, one needs to know the rate and the molecular spectrum of spontaneous …

Although mutation provides the fuel for phenotypic evolution, it also imposes a substantial
burden on fitness through the production of predominantly deleterious alleles, a matter of …

Adaptation from standing genetic variation or recurrent de novo mutation in large
populations should commonly generate soft rather than hard selective sweeps. In contrast to …