Although transcription is an essential cellular process, it is paradoxically also a well-
recognized cause of genomic instability. R-loops, non-B DNA structures formed when …

From bacteria to mammalian cells, damaged DNA is sensed and targeted by DNA repair
pathways. In eukaryotes, kinases play a central role in coordinating the DNA damage …

R-loops have both positive and negative impacts on chromosome functions. To identify toxic
R-loops in the human genome, here, we map RNA: DNA hybrids, replication stress markers …

Collisions between the DNA replication machinery and co-transcriptional R-loops can
impede DNA synthesis and are a major source of genomic instability in cancer cells. How …

During each cell duplication, the entirety of the genomic DNA in every cell must be
accurately and quickly copied. Given the short time available for the chore, the requirement …

DNA replicates once per cell cycle. Interfering with the regulation of DNA replication
initiation generates genome instability through over-replication and has been linked to early …

Transcription-replication conflict is a major cause of replication stress that arises when
replication forks collide with the transcription machinery. Replication fork stalling at sites of …

Summary The Mec1 and Rad53 kinases play a central role during acute replication stress in
budding yeast. They are also essential for viability in normal growth conditions, but the …

The Rad53 DNA checkpoint protein kinase plays multiple roles in the budding yeast cell
response to DNA replication stress. Key amongst these is its enigmatic role in safeguarding …

Assembly of TopBP1 biomolecular condensates triggers activation of the ataxia
telangiectasia-mutated and Rad3-related (ATR)/Chk1 signaling pathway, which coordinates …