Bioinspired, Size‐Tunable Self‐Assembly of Polymer–Lipid Bilayer Nanodiscs

T Ravula, SK Ramadugu, G Di Mauro… - Angewandte …, 2017 - Wiley Online Library
Polymer‐based nanodiscs are valuable tools in biomedical research that can offer a
detergent‐free solubilization of membrane proteins maintaining their native lipid …

Formation of pH‐Resistant Monodispersed Polymer–Lipid Nanodiscs

T Ravula, NZ Hardin, SK Ramadugu… - Angewandte Chemie …, 2018 - Wiley Online Library
Polymer lipid nanodiscs are an invaluable system for structural and functional studies of
membrane proteins in their near‐native environment. Despite the recent advances in the …

Formation of lipid-bilayer nanodiscs by diisobutylene/maleic acid (DIBMA) copolymer

AO Oluwole, J Klingler, B Danielczak, JO Babalola… - Langmuir, 2017 - ACS Publications
Membrane proteins usually need to be extracted from their native environment and
separated from other membrane components for in-depth in vitro characterization. The use …

Synthesis, Characterization, and Nanodisc Formation of Non‐ionic Polymers

T Ravula, A Ramamoorthy - Angewandte Chemie, 2021 - Wiley Online Library
Although lipid nanodiscs are increasingly used in the structural studies of membrane
proteins, drug delivery and other applications, the interaction between the nanodisc belt and …

Magnetic Alignment of Polymer Macro‐Nanodiscs Enables Residual‐Dipolar‐Coupling‐Based High‐Resolution Structural Studies by NMR Spectroscopy

T Ravula, A Ramamoorthy - Angewandte Chemie, 2019 - Wiley Online Library
Experimentally measured residual dipolar couplings (RDCs) are highly valuable for atomic‐
resolution structural and dynamic studies of molecular systems ranging from small …

Metal‐Chelated Polymer Nanodiscs for NMR Studies

NZ Hardin, V Kocman, GM Di Mauro… - Angewandte Chemie …, 2019 - Wiley Online Library
Paramagnetic relaxation enhancement (PRE) is commonly used to speed up spin lattice
relaxation time (T1) for rapid data acquisition in NMR structural studies. Consequently, there …

A Diazirine‐Modified Membrane Lipid to Study Peptide/Lipid Interactions–Chances and Challenges

J Dorner, P Korn, K Gruhle, D Ramsbeck… - … A European Journal, 2021 - Wiley Online Library
Although incorporation of photo‐activatable lipids into membranes potentially opens up
novel avenues for investigating interactions with proteins, the question of whether diazirine …

Detergent‐Like Polymerizable Monomers: Synthesis, Physicochemical, and Biochemical Characterization

C Bonnet, P Guillet, F Mahler, S Igonet… - European Journal of …, 2020 - Wiley Online Library
Three monomers with a maltose polar head, an alkyl hydrogenated chain, and an
acrylamide‐based polymerizable moiety were synthesized. The self‐assembly properties in …