Harnessing microfluidic technology for bacterial single-cell analysis in mammals

X Guo, Q Shen, Z Chen, Z He, X Yan - TrAC Trends in Analytical Chemistry, 2023 - Elsevier
Bacteria, as the predominant species in mammalian microbiomes, play important roles in the
regulation of host health and disease. Bacterial analysis down to the single-cell level is …

Fluorogenic Probes of the Mycobacterial Membrane as Reporters of Antibiotic Action

MG Wuo, CL Dulberger, TC Warner… - Journal of the …, 2024 - ACS Publications
The genus Mycobacterium includes species such as Mycobacterium tuberculosis, which can
cause deadly human diseases. These bacteria have a protective cell envelope that can be …

The contribution of microfluidics to the fight against tuberculosis

M Cañadas-Ortega, C Gómez-Cruz… - Nanotechnology …, 2021 - degruyter.com
The high mortality associated with tuberculosis brings forward the urgency of developing
new therapies and strategies against the disease. With the advance of drug-resistant strains …

Antibiotic action revealed by real-time imaging of the mycobacterial membrane

MG Wuo, CL Dulberger, RA Brown, A Sturm, E Ultee… - Biorxiv, 2022 - biorxiv.org
The current understanding of mycobacterial cell envelope remodeling in response to
antibiotics is limited. Chemical tools that report on phenotypic changes with minimal cell wall …

Revealing Antibiotic Tolerance of the Mycobacterium smegmatis Xanthine/Uracil Permease Mutant Using Microfluidics and Single-Cell Analysis

M Elitas, N Dhar, JD McKinney - Antibiotics, 2021 - mdpi.com
To reveal rare phenotypes in bacterial populations, conventional microbiology tools should
be advanced to generate rapid, quantitative, accurate, and high-throughput data. The main …

[HTML][HTML] Mycobacterium smegmatis causing a granulomatous cardiomediastinal mass

MM Alqurashi, A Alsaileek, A Aljizeeri, HS Bamefleh… - IDCases, 2019 - Elsevier
Non-tuberculous mycobacterial species are uncommon human pathogens. They are divided
into slow and rapid growing mycobacteria (RGM) with Mycobacterium smegmatis group as …

On-Chip Isoniazid Exposure of Mycobacterium smegmatis Penicillin-Binding Protein (PBP) Mutant Using Time-Lapse Fluorescent Microscopy

M Elitas - Micromachines, 2018 - mdpi.com
Antibiotic resistance has been one of the biggest threats to global health. Despite the
available prevention and control strategies and efforts in developing new antibiotics, the …

The first comprehensive phylogenetic and biochemical analysis of NADH diphosphatases reveals that the enzyme from Tuber melanosporum is highly active towards …

AG García-Saura, R Zapata-Pérez… - Scientific Reports, 2019 - nature.com
Nudix (for nu cleoside di phosphatases linked to other moieties, X) hydrolases are a diverse
family of proteins capable of cleaving an enormous variety of substrates, ranging from …

[PDF][PDF] Chapter-10 Time-Lapse Imaging for Rapid TB Drug Screening

S Biswal, D Sinha, AK Mohapatra - MEDICAL, 2023 - researchgate.net
Time-lapse systems have quickly become a common feature of drug screening all over the
world. Since being introduced over a decade ago, the claimed benefits of time-lapse …

[PDF][PDF] A Review of Time-Lapse Imaging on TB Drug Screening

AK Mohapatra, A Priyadarshini - Available at SSRN 4084465, 2022 - papers.ssrn.com
Time-lapse systems have quickly become a common feature of drug screening all over the
world. Since being introduced over a decade ago, the claimed benefits of time-lapse …