Biomolecular condensates (BMCs) can facilitate or inhibit diverse cellular functions. BMC
formation is driven by noncovalent protein–protein, protein–RNA, and RNA–RNA …

ABSTRACT Introduction Intrinsically disordered proteins (IDPs) represent a family of
proteins that lack secondary or tertiary structure. IDPs are hubs in interaction networks …

Protein arginine methyltransferases (PRMTs) catalyze the post-translational
monomethylation (Rme1), asymmetric (Rme2a), or symmetric (Rme2s) dimethylation of …

Posttranslational modifications (PTMs) such as phosphorylation of RNA-binding proteins
(RBPs) regulate several critical steps in RNA metabolism, including spliceosome assembly …

Fragmentation trends of large peptides were characterized by five activation methods,
including HCD, ETD, EThcD, 213 nm UVPD, and 193 nm UVPD. Sequence coverages and …

Early spliceosome assembly requires phosphorylation of U1-70K, a constituent of the U1
small nuclear ribonucleoprotein (snRNP), but it is unclear which sites are phosphorylated …

Core spliceosome and related RNA-binding proteins aggregate in Alzheimer's disease (AD)
brain even in early asymptomatic stages (AsymAD) of disease. To assess the specificity of …

Protein arginine methylations are important post-translational modifications (PTMs) in
eukaryotes, regulating many biological processes. However, traditional collision-based …

The formation of condensates in membraneless organelles is thought to be driven by protein
phase separation. Arginine methylation and serine/threonine phosphorylation are important …

Middle‐down proteomics (MDP) is an analytical approach in which protein samples are
digested with proteases such as Glu‐C to generate large peptides (> 3 kDa) that are …