CRISPR–Cas9 delivery strategies for the modulation of immune and non-immune cells
CRISPR–Cas9 genome editing technology is a promising tool for genetically engineering
immune cells and modulating immune systems. Although ex vivo genome editing of immune …
immune cells and modulating immune systems. Although ex vivo genome editing of immune …
Peptide-based self-assembled monolayers (SAMs): what peptides can do for SAMs and vice versa
C Redondo-Gómez, P Parreira, MCL Martins… - Chemical Society …, 2024 - pubs.rsc.org
Self-assembled monolayers (SAMs) represent highly ordered molecular materials with
versatile biochemical features and multidisciplinary applications. Research on SAMs has …
versatile biochemical features and multidisciplinary applications. Research on SAMs has …
Targeted nonviral delivery of genome editors in vivo
CA Tsuchida, KM Wasko… - Proceedings of the …, 2024 - National Acad Sciences
Cell-type-specific in vivo delivery of genome editing molecules is the next breakthrough that
will drive biological discovery and transform the field of cell and gene therapy. Here, we …
will drive biological discovery and transform the field of cell and gene therapy. Here, we …
Engineering self-deliverable ribonucleoproteins for genome editing in the brain
The delivery of CRISPR ribonucleoproteins (RNPs) for genome editing in vitro and in vivo
has important advantages over other delivery methods, including reduced off-target and …
has important advantages over other delivery methods, including reduced off-target and …
Harnessing the evolving CRISPR/Cas9 for precision oncology
T Li, S Li, Y Kang, J Zhou, M Yi - Journal of Translational Medicine, 2024 - Springer
Abstract The Clustered Regularly Interspaced Short Palindromic Repeat (CRISPR)/Cas9
system, a groundbreaking innovation in genetic engineering, has revolutionized our …
system, a groundbreaking innovation in genetic engineering, has revolutionized our …
Gene editing without ex vivo culture evades genotoxicity in human hematopoietic stem cells
Gene editing the BCL11A erythroid enhancer is a validated approach to fetal hemoglobin
(HbF) induction for β-hemoglobinopathy therapy, though heterogeneity in edit allele …
(HbF) induction for β-hemoglobinopathy therapy, though heterogeneity in edit allele …
Applications of CRISPR technology in cellular immunotherapy
CRISPR technology has transformed multiple fields, including cancer and immunology.
CRISPR‐based gene editing and screening empowers direct genomic manipulation of …
CRISPR‐based gene editing and screening empowers direct genomic manipulation of …
Leveraging CRISPR gene editing technology to optimize the efficacy, safety and accessibility of CAR T-cell therapy
T Lei, Y Wang, Y Zhang, Y Yang, J Cao, J Huang… - Leukemia, 2024 - nature.com
Abstract Chimeric Antigen Receptor (CAR)-T-cell therapy has revolutionized cancer immune
therapy. However, challenges remain including increasing efficacy, reducing adverse events …
therapy. However, challenges remain including increasing efficacy, reducing adverse events …
Amphipathic cell-penetrating peptide-aided delivery of Cas9 RNP for in vitro gene editing and correction
The therapeutic potential of the CRISPR-Cas9 gene editing system in treating numerous
genetic disorders is immense. To fully realize this potential, it is crucial to achieve safe and …
genetic disorders is immense. To fully realize this potential, it is crucial to achieve safe and …
Neuronal lineage tracing from progenitors in human cortical organoids reveals mechanisms of neuronal production, diversity, and disease
LAD Bury, S Fu, A Wynshaw-Boris - Cell Reports, 2024 - cell.com
The contribution of progenitor subtypes to generating the billions of neurons produced
during human cortical neurogenesis is not well understood. We developed the cortical …
during human cortical neurogenesis is not well understood. We developed the cortical …