Life depends on the faithful translation of the genetic information into proteins. Ribosomes
have developed remarkable mechanisms to ensure the accurate synthesis of proteins. In the …

Nearly half of the ribosomes translating a particular bacteriophage T4 mRNA bypass a
region of 50 nt, resuming translation 3′ of this gap. How this large-scale, specific hop …

酶在医疗和生物药物方面有着广泛的应用, 不仅可以用来治疗各种疾病, 还在临床诊断和人体
健康等方面有着重要的影响. 利用微生物来表达异源蛋白已经成为获取酶最简单快速的方法 …

During mRNA translation, synonymous codons for one amino acid are often read by different
isoaccepting tRNAs. The theory of selective tRNA charging predicts greatly varying …

Ribosome profiling spectra bear rich information on translation control and dynamics. Yet,
due to technical biases in library generation, extracting quantitative measures of discrete …

Ribosome-mediated transcriptional attenuation mechanisms are commonly used to control
amino acid biosynthetic operons in bacteria. The mRNA leader of such an operon contains …

Molecular biology emerged through unification of genetics and nucleic acid chemistry that
took place with the discovery of the double helix (Watson and Crick 1953). Accordingly …

The versatility of the cellYfree system has provided the additional possibility to study the
effects of class II release factor RF3 mutations in mediating fast dissociation of class I …

I will discuss a theoretical model for how the charging levels of different tRNA isoacceptors
respond when supply of their amino acid becomes growth limiting [1]. The charged levels of …

During decoding of the genetic message, the ribosome must select the correct substrate, a
ternary complex aminoacyl‐tRNA–EF‐Tu–GTP, from a large number of almost identical …