Recent advances in luminescent materials for super-resolution imaging via stimulated emission depletion nanoscopy

Y Xu, R Xu, Z Wang, Y Zhou, Q Shen, W Ji… - Chemical Society …, 2021 - pubs.rsc.org
Stimulated emission depletion (STED) nanoscopy is a promising fluorescence microscopy to
detect unresolvable structures at the nanoscale level and then achieve a superior imaging …

Seeing beyond the limit: A guide to choosing the right super-resolution microscopy technique

J Valli, A Garcia-Burgos, LM Rooney… - Journal of Biological …, 2021 - ASBMB
Super-resolution microscopy has become an increasingly popular and robust tool across the
life sciences to study minute cellular structures and processes. However, with the increasing …

Universal quenching of common fluorescent probes by water and alcohols

J Maillard, K Klehs, C Rumble, E Vauthey… - Chemical …, 2021 - pubs.rsc.org
Although biological imaging is mostly performed in aqueous media, it is hardly ever
considered that water acts as a classic fluorescence quencher for organic fluorophores. By …

Stimulated emission depletion microscopy

H Blom, J Widengren - Chemical reviews, 2017 - ACS Publications
Despite its short history, diffraction-unlimited fluorescence microscopy techniques have
already made a substantial imprint in the biological sciences. In this review, we describe …

Choose your label wisely: water-soluble fluorophores often interact with lipid bilayers

LD Hughes, RJ Rawle, SG Boxer - PloS one, 2014 - journals.plos.org
Water-soluble organic fluorophores are widely used as labels in biological systems.
However, in many cases these fluorophores can interact strongly with lipid bilayers …

Coaligned dual-channel STED nanoscopy and molecular diffusion analysis at 20 nm resolution

F Göttfert, CA Wurm, V Mueller, S Berning, VC Cordes… - Biophysical journal, 2013 - cell.com
We report on a fiber laser-based stimulated emission-depletion microscope providing down
to∼ 20 nm resolution in raw data images as well as 15–19 nm diameter probing areas in …

Seeing the unseen: AIE luminogens for super-resolution imaging

Z Wang, Y Zhou, R Xu, Y Xu, D Dang, Q Shen… - Coordination Chemistry …, 2022 - Elsevier
Super-resolution imaging (SRI) is of great significance to break the Abbe's diffraction limit,
enabling visualization and dynamic monitoring with ultra-high resolution in both material …

Fluorescent dyes with large Stokes shifts for super-resolution optical microscopy of biological objects: a review

MV Sednev, VN Belov, SW Hell - Methods and applications in …, 2015 - iopscience.iop.org
The review deals with commercially available organic dyes possessing large Stokes shifts
and their applications as fluorescent labels in optical microscopy based on stimulated …

Lens-based fluorescence nanoscopy

C Eggeling, KI Willig, SJ Sahl, SW Hell - Quarterly reviews of …, 2015 - cambridge.org
The majority of studies of the living cell rely on capturing images using fluorescence
microscopy. Unfortunately, for centuries, diffraction of light was limiting the spatial resolution …

[HTML][HTML] Twinkle, twinkle little star: Photoswitchable fluorophores for super-resolution imaging

TJ Chozinski, LA Gagnon, JC Vaughan - FEBS letters, 2014 - Elsevier
Photoswitchable fluorescent probes are key elements of newly developed super-resolution
fluorescence microscopy techniques that enable far-field interrogation of biological systems …