Homologous recombination (HR) is a central process to ensure genomic stability in somatic
cells and during meiosis. HR-associated DNA synthesis determines in large part the fidelity …

DNA polymerases synthesize DNA in only one direction, but large genomes require RNA
priming and bidirectional replication from internal origins. We review here the physical …

In eukaryotes, DNA polymerase δ (Pol δ) bound to the proliferating cell nuclear antigen
(PCNA) replicates the lagging strand and cooperates with flap endonuclease 1 (FEN1) to …

During DNA repair by homologous recombination (HR), DNA synthesis copies information
from a template DNA molecule. Multiple DNA polymerases have been implicated in repair …

Genetic studies with S. cerevisiae Polδ (pol3-L612M) and Polε (pol2-M644G) mutant alleles,
each of which display a higher rate for the generation of a specific mismatch, have led to the …

DNA polymerase ζ (Pol ζ) plays a key role in DNA translesion synthesis (TLS) and
mutagenesis in eukaryotes. Previously, a two-subunit Rev3–Rev7 complex had been …

Replication through a diverse array of DNA lesions occurs by the sequential action of two
translesion synthesis (TLS) DNA polymerases (Pols), in which one inserts the nucleotide …

Break-induced replication (BIR) is a pathway of homology-directed repair that repairs one-
ended DNA breaks, such as those formed at broken replication forks or uncapped …

Translesion DNA synthesis is an important branch of the DNA damage tolerance pathway
that assures genomic integrity of living organisms. The mechanisms of DNA polymerase …

Pif1 DNA helicase is a potent unwinder of G-quadruplex (G4) structures in vitro and
functions to maintain genome stability at G4 sequences in Saccharomyces cerevisiae. Here …