DNA amplification is required for most molecular diagnostic applications, but conventional
polymerase chain reaction (PCR) has disadvantages for field testing. Isothermal …

The objective of the present study was to establish a system of real-time polymerase chain
reactions (PCRs) for the specific detection of Yersinia pestis using the LightCycler™(LC) …

Plague, caused by the Yersinia pestis bacterium, has several foci scattered throughout a
large area from the Brazilian territory that ranges from the Northeastern State of Ceará to the …

La introducción de métodos de biología molecular en los laboratorios de microbiología
clínica supone un gran apoyo a la hora de obtener diagnósticos sensibles y específicos en …

Yersinia pestis, the aetiological agent of the plague, causes sporadic disease in endemic
areas of the world and is classified as a National Institute of Allergy and Infectious Diseases …

A multiplex real‐time polymerase chain reaction (PCR) assay was developed for the
detection of Yersinia pestis and Yersinia pseudotuberculosis. The assay includes four …

Plague is an acute bacterial infection caused by Yersinia pestis. The three major clinical
forms of plague are bubonic, pneumonic and septicemic, which have high case‑mortality …

Materials and Methods The specificity of the assay was tested using reference strains of
vancomycin-resistant and susceptible enterococci. In total, 193 clinical isolates were …

Background: Yersinia pestis, the causative agent of the zoonotic infection plague, is a major
concern as a potential bioweapon. Current real-time PCR assays used for Y. pestis …

In Brazil, plague is a greatly neglected disease. It received some attention when it was first
introduced in 1899 and again during the first decades of the twenty century, when it spread …