Chromatin accessibility profiling by ATAC-seq
The assay for transposase-accessible chromatin using sequencing (ATAC-seq) provides a
simple and scalable way to detect the unique chromatin landscape associated with a cell …
simple and scalable way to detect the unique chromatin landscape associated with a cell …
Transcriptional enhancers: from properties to genome-wide predictions
Cellular development, morphology and function are governed by precise patterns of gene
expression. These are established by the coordinated action of genomic regulatory …
expression. These are established by the coordinated action of genomic regulatory …
Epigenomic signatures of neuronal diversity in the mammalian brain
Neuronal diversity is essential for mammalian brain function but poses a challenge to
molecular profiling. To address the need for tools that facilitate cell-type-specific epigenomic …
molecular profiling. To address the need for tools that facilitate cell-type-specific epigenomic …
The Drosophila embryo at single-cell transcriptome resolution
N Karaiskos, P Wahle, J Alles, A Boltengagen, S Ayoub… - Science, 2017 - science.org
By the onset of morphogenesis, Drosophila embryos consist of about 6000 cells that express
distinct gene combinations. Here, we used single-cell sequencing of precisely staged …
distinct gene combinations. Here, we used single-cell sequencing of precisely staged …
Site-specific biotinylation of purified proteins using BirA
M Fairhead, M Howarth - Site-Specific Protein Labeling: Methods and …, 2015 - Springer
The binding between biotin and streptavidin or avidin is one of the strongest known non-
covalent biological interactions. The (strept) avidin-biotin interaction has been widely used …
covalent biological interactions. The (strept) avidin-biotin interaction has been widely used …
An expanded auxin-inducible degron toolkit for Caenorhabditis elegans
GE Ashley, T Duong, MT Levenson, MAQ Martinez… - Genetics, 2021 - academic.oup.com
The auxin-inducible degron (AID) system has emerged as a powerful tool to conditionally
deplete proteins in a range of organisms and cell types. Here, we describe a toolkit to …
deplete proteins in a range of organisms and cell types. Here, we describe a toolkit to …
Cell-type-specific profiling of gene expression and chromatin binding without cell isolation: assaying RNA Pol II occupancy in neural stem cells
TD Southall, KS Gold, B Egger, CM Davidson… - Developmental cell, 2013 - cell.com
Cell-type-specific transcriptional profiling often requires the isolation of specific cell types
from complex tissues. We have developed" TaDa," a technique that enables cell-specific …
from complex tissues. We have developed" TaDa," a technique that enables cell-specific …
Temporal transitions in the post-mitotic nervous system of Caenorhabditis elegans
In most animals, the majority of the nervous system is generated and assembled into
neuronal circuits during embryonic development. However, during juvenile stages, nervous …
neuronal circuits during embryonic development. However, during juvenile stages, nervous …
Simultaneous transcriptional and epigenomic profiling from specific cell types within heterogeneous tissues in vivo
Epigenomic mechanisms direct distinct gene expression programs for different cell types.
Various in vivo tissues have been subjected to epigenomic analysis; however, these studies …
Various in vivo tissues have been subjected to epigenomic analysis; however, these studies …
Cell-type-specific profiling of protein–DNA interactions without cell isolation using targeted DamID with next-generation sequencing
This protocol is an extension to: Nat. Protoc. 2, 1467–1478 (2007); doi: 10.1038/nprot.
2007.148; published online 7 June 2007 The ability to profile transcription and chromatin …
2007.148; published online 7 June 2007 The ability to profile transcription and chromatin …