Auto-induction medium for the production of [U-15N]-and [U-13C, U-15N]-labeled proteins for NMR screening and structure determination

RC Tyler, HK Sreenath, S Singh, DJ Aceti… - Protein expression and …, 2005 - Elsevier
Protocols have been developed and applied for the high-throughput production of [U-15N]-
or [U-13C-, U-15N]-labeled proteins using the conditional methionine auxotroph Escherichia …

Protocols for production of selenomethionine-labeled proteins in 2-L polyethylene terephthalate bottles using auto-induction medium

HK Sreenath, CA Bingman, BW Buchan… - Protein expression and …, 2005 - Elsevier
Protocols have been developed and applied in the high-throughput production of
selenomethionine labeled fusion proteins using the conditional Met auxotroph Escherichia …

Comparison of cell‐based and cell‐free protocols for producing target proteins from the Arabidopsis thaliana genome for structural studies

RC Tyler, DJ Aceti, CA Bingman… - Proteins: Structure …, 2005 - Wiley Online Library
We describe a comparative study of protein production from 96 Arabidopsis thaliana open
reading frames (ORFs) by cell‐based and cell‐free protocols. Each target was carried …

X-ray structure of Arabidopsis At2g06050, 12-oxophytodienoate reductase isoform 3

TE Malone, SE Madson, RL Wrobel, WB Jeon… - …, 2005 - pubmed.ncbi.nlm.nih.gov
X-ray structure of Arabidopsis At2g06050, 12-oxophytodienoate reductase isoform 3 X-ray
structure of Arabidopsis At2g06050, 12-oxophytodienoate reductase isoform 3 Proteins. 2005 …

Fluorescence anisotropy assay for proteolysis of specifically labeled fusion proteins

PG Blommel, BG Fox - Analytical biochemistry, 2005 - Elsevier
A cloning method and plasmid vectors that permit fluorescence-anisotropy-based
measurement of proteolysis are reported. The recombinant protein substrates produced by …

[PDF][PDF] Crystal structure of At2g03760, a putative steroid sulfotransferase from Arabidopsis thaliana

DW Smith, KA Johnson, CA Bingman… - … Structure Function and …, 2004 - academia.edu
Materials and Methods. Callus tissue from an Arabidopsis thaliana Ecotype Columbia T87
suspension culture was collected by filtration. The callus was then ground to a fine powder …

Solution structure of At3g04780.1‐des15, an Arabidopsis thaliana ortholog of the C‐terminal domain of human thioredoxin‐like protein

J Song, RC Tyler, RL Wrobel, RO Frederick… - Protein …, 2005 - Wiley Online Library
The structure of At3g04780. 1‐des15, an Arabidopsis thaliana ortholog of the C‐terminal
domain of human thioredoxin‐like protein, was determined by NMR spectroscopy. The …

Solution structure of a calmodulin-like calcium-binding domain from Arabidopsis thaliana

J Song, Q Zhao, S Thao, RO Frederick… - Journal of biomolecular …, 2004 - Citeseer
The diffusion coefficient of Ca-nCML at various concentrations has been determined by the
water-suppressed longitudinal encode-decode (Water-SLED) experiment (Altieri et al …

X‐ray structure of Arabidopsis At1g77680, 12‐oxophytodienoate reductase isoform 1

BG Fox, TE Malone, KA Johnson… - Proteins: Structure …, 2005 - Wiley Online Library
Materials and Methods. The cDNA preparation used for cloning was obtained by conversion
of the total mRNA isolated from an Arabidopsis tissue culture using reverse transcriptase. 7 …

[PDF][PDF] Crystal structure of the protein from gene At3g17210 of Arabidopsis thaliana

CA Bingman, KA Johnson, FC Peterson… - PROTEINS: Structure …, 2004 - academia.edu
Introduction. The Center for Eukaryotic Structural Genomics (CESG) is dedicated to
determining the structures of novel proteins from Arabidopsis thaliana. Proteins, or at least …