The fascinating biology behind phage display: filamentous phage assembly

B Loh, A Kuhn, S Leptihn - Molecular Microbiology, 2019 - Wiley Online Library
With the recently awarded Nobel Prize to the inventor of Phage Display, George Smith, the
technique has once more gained attention. However, one should not forget about the …

Discovery of several thousand highly diverse circular DNA viruses

MJ Tisza, DV Pastrana, NL Welch, B Stewart, A Peretti… - elife, 2020 - elifesciences.org
Although millions of distinct virus species likely exist, only approximately 9000 are
catalogued in GenBank's RefSeq database. We selectively enriched for the genomes of …

From cholera to corals: viruses as drivers of virulence in a major coral bacterial pathogen

KD Weynberg, CR Voolstra, MJ Neave, P Buerger… - Scientific reports, 2015 - nature.com
Disease is an increasing threat to reef-building corals. One of the few identified pathogens of
coral disease is the bacterium Vibrio coralliilyticus. In Vibrio cholerae, infection by a bacterial …

Molecular mechanism of acquisition of the cholera toxin genes

B Das, J Bischerour, FX Barre - Indian Journal of Medical …, 2011 - journals.lww.com
One of the major pathogenic determinants of Vibrio cholerae, the cholera toxin, is encoded
in the genome of a filamentous phage, CTXφ. CTXφ makes use of the chromosome dimer …

VEJφ, a novel filamentous phage of Vibrio cholerae able to transduce the cholera toxin genes

J Campos, E Martínez, Y Izquierdo… - Microbiology, 2010 - microbiologyresearch.org
A novel filamentous bacteriophage, designated VEJ φ, was isolated from strain MO45 of
Vibrio cholerae of the O139 serogroup. A molecular characterization of the phage was …

Effect of LexA on chromosomal integration of CTXϕ in Vibrio cholerae

A Pant, D Anbumani, S Bag, O Mehta… - Journal of …, 2016 - Am Soc Microbiol
The genesis of toxigenic Vibrio cholerae involves acquisition of CTXϕ, a single-stranded
DNA (ssDNA) filamentous phage that encodes cholera toxin (CT). The phage exploits host …

A novel, broad-range, CTXΦ-derived stable integrative expression vector for functional studies

B Das, R Kumari, A Pant, S Sen Gupta… - Journal of …, 2014 - Am Soc Microbiol
ABSTRACT CTXΦ, a filamentous vibriophage encoding cholera toxin, uses a unique
strategy for its lysogeny. The single-stranded phage genome forms intramolecular base …

[HTML][HTML] Acquisition and dissemination mechanisms of CTXΦ in Vibrio cholerae: New paradigm for dif residents

B Das, GB Nair, RK Bhadra - World Journal of Medical Genetics, 2014 - wjgnet.com
Vibrio cholerae (V. cholerae) genome is equipped with a number of integrative mobile
genetic element (IMGE) like prophages, plasmids, transposons or genomic islands, which …

Characterization of the single-stranded DNA binding protein pVVGJΦ of VGJΦ phage from Vibrio cholerae

A Falero, A Caballero, S Trigueros, C Pérez… - … et Biophysica Acta (BBA …, 2011 - Elsevier
Abstract pV VGJΦ, a single-stranded DNA binding protein of the vibriophage VGJΦ was
subject to biochemical analysis. Here, we show that this protein has a general affinity for …

The genomics of cholera

B Das, GB Nair - Genomics Applications for the Developing World, 2012 - Springer
Vibrio cholerae, a bacterium autochthonous to the aquatic environment and introduced into
the human intestine through contaminated water or food, is the etiological agent of the acute …