Human blood is conventionally considered sterile but recent studies suggest the presence of
a blood microbiome in healthy individuals. Here we characterized the DNA signatures of …

Human blood contains low biomass of circulating microbial cell-free DNA (cfmDNA) that
predominantly originates from bacteria. Numerous studies have detected circulating …

Next‐generation sequencing (NGS) has developed rapidly in the last decade and is
emerging as a promising diagnostic tool for periprosthetic joint infection (PJI). However, its …

Metagenomic next-generation sequencing (mNGS) and droplet digital PCR (ddPCR) have
recently demonstrated a great potential for pathogen detection. However, few studies have …

Background Microbial cell-free DNA (mcfDNA) sequencing of plasma can identify the
presence of a pathogen in a host. In this study, we evaluated the duration of pathogen …

The increasing incidence of bloodstream infections including sepsis is a major challenge in
intensive care units worldwide. However, current diagnostics for pathogen identification …

The performance of metagenomic next-generation sequencing (mNGS) was evaluated and
compared with that of conventional culture testing in patients with sepsis. Prospective blood …

Background Conventional blood cultures were compared to plasma cell-free DNA–based
16S ribosomal RNA (rRNA) gene polymerase chain reaction (PCR)/next-generation …

Background In this study, we aimed to perform a comprehensive analysis on the
metagenomic next-generation sequencing for the etiological diagnosis of septic patients …

The application of metagenomic next-generation sequencing (mNGS) has gradually been
carried out by clinical practitioner. However, few studies have compared it with blood …