Cell wall hydrolases in bacteria: insight on the diversity of cell wall amidases, glycosidases and peptidases toward peptidoglycan

A Vermassen, S Leroy, R Talon, C Provot… - Frontiers in …, 2019 - frontiersin.org
The cell wall (CW) of bacteria is an intricate arrangement of macromolecules, at least
constituted of peptidoglycan (PG) but also of (lipo) teichoic acids, various polysaccharides …

Cell-wall recycling of the gram-negative bacteria and the nexus to antibiotic resistance

DA Dik, JF Fisher, S Mobashery - Chemical reviews, 2018 - ACS Publications
The importance of the cell wall to the viability of the bacterium is underscored by the breadth
of antibiotic structures that act by blocking key enzymes that are tasked with cell-wall …

Constructing and deconstructing the bacterial cell wall

JF Fisher, S Mobashery - Protein science, 2020 - Wiley Online Library
The history of modern medicine cannot be written apart from the history of the antibiotics.
Antibiotics are cytotoxic secondary metabolites that are isolated from Nature. The …

Functional metagenomic screening identifies an unexpected β-glucuronidase

S Neun, P Brear, E Campbell, T Tryfona… - Nature Chemical …, 2022 - nature.com
The abundance of recorded protein sequence data stands in contrast to the small number of
experimentally verified functional annotation. Here we screened a million-membered …

[HTML][HTML] Dissecting conformational contributions to glycosidase catalysis and inhibition

G Speciale, AJ Thompson, GJ Davies… - Current opinion in …, 2014 - Elsevier
Highlights•The conformational itinerary describes the changes in sugar shape during
catalysis.•Stereoelectronic requirements for glycoside hydrolysis are discussed.•Major and …

Enzymatic breakdown of lignocellulosic biomass: the role of glycosyl hydrolases and lytic polysaccharide monooxygenases

UR Ezeilo, II Zakaria, F Huyop… - Biotechnology & …, 2017 - Taylor & Francis
Lignocellulose constitutes a major component of discarded wastes from various industries
viz. agriculture, forestry and municipal waste treatment. The potential use of lignocellulose …

The β-lactamase gene regulator AmpR is a tetramer that recognizes and binds the D-Ala-D-Ala motif of its repressor UDP-N-acetylmuramic acid (MurNAc) …

G Vadlamani, MD Thomas, TR Patel, LJ Donald… - Journal of biological …, 2015 - ASBMB
Inducible expression of chromosomal AmpC β-lactamase is a major cause of β-lactam
antibiotic resistance in the Gram-negative bacteria Pseudomonas aeruginosa and …

The evolutionary advantage of an aromatic clamp in plant family 3 glycoside exo-hydrolases

S Luang, X Fernández-Luengo, A Nin-Hill… - Nature …, 2022 - nature.com
In the barley β-d-glucan glucohydrolase, a glycoside hydrolase family 3 (GH3) enzyme, the
Trp286/Trp434 clamp ensures β-d-glucosides binding, which is fundamental for substrate …

Aspergillus niger β-glucosidase has a cellulase-like tadpole molecular shape: insights into glycoside hydrolase family 3 (GH3) β-glucosidase structure and function

MA Lima, M Oliveira-Neto, MAS Kadowaki… - Journal of Biological …, 2013 - ASBMB
Aspergillus niger is known to secrete large amounts of β-glucosidases, which have a variety
of biotechnological and industrial applications. Here, we purified an A. niger β-glucosidase …

Improvements of the productivity and saccharification efficiency of the cellulolytic β-glucosidase D2-BGL in Pichia pastoris via directed evolution

MR Kao, SM Yu, THD Ho - Biotechnology for Biofuels, 2021 - Springer
Background β-Glucosidases are essential for cellulose hydrolysis by catalyzing the final
cellulolytic degradation of cello-oligomers and cellobiose to glucose. D2-BGL is a fungal …