Developing recombinant protein pharmaceuticals has proved to be very challenging
because of both the complexity of protein production and purification, and the limited …

Publisher Summary Hydration can be considered a process of adding water incrementally to
dry protein, until a level of hydration is reached beyond which further addition of water …

Materials and Methods Materials. Hen egg white lysozyme (HC1) was purchased from
Boehringer-Mannheim and bovine serum albumin (BSA) 1 from Sigma. These proteins were …

This review deals with dynamics of water molecules in the hydration layer that surrounds
selfassemblies and proteins in aqueous solutions. This topic has not only seen a vigorous …

The nature of the interaction responsible for the inhibition of protein unfolding and
subsequent damage by sugars during dehydration is unclear. The relationship between …

Attenuated total re£ ection Fourier transform infrared spectroscopy (ATR-FTIR) is one of the
most powerful methods for recording infrared spectra of biological materials in general, and …

Enzyme catalysis in organic solvents is being increasingly used for a variety of applications.
Of special interest are the cases in which the medium is predominantly non‐aqueous and …

▪ Abstract Although protein function is thought to depend on flexibility, precisely how the
dynamics of the molecule and its environment contribute to catalytic mechanisms is unclear …

Cellular organization and the overall biochemistry of an organism depend on water, if only
because on a molecular level water determines the reaction rates and thermodynamics of …

Changes in the secondary structure of some dozen different proteins upon lyophilization of
their aqueous solutions have been investigated by means of Fourier-transform infrared …