For over three decades, Chinese hamster ovary (CHO) cells have been the chosen
expression platform for the production of therapeutic proteins with complex post …

The N-linked glycan profiles on recombinant monoclonal antibody therapeutics significantly
affect antibody biological functions and are largely determined by host cell genotypes and …

Chinese hamster ovary (CHO) cells dominate biotherapeutic protein production and are
widely used in mammalian cell line engineering research. To elucidate metabolic …

Through process transfer and optimization for increased antibody production to 3 g/L for a
GS‐CHO cell line, an undesirable drop in antibody Fc galactosylation was observed. Uridine …

The glycosylation profile of therapeutic antibodies is routinely analyzed throughout
development to monitor the impact of process parameters and to ensure consistency …

The addition of nutrients and accumulation of metabolites in a fed-batch culture of Chinese
hamster ovary (CHO) cells leads to an increase in extracellular osmolality in late stage …

Amino acids are crucial for the cultivation of mammalian cells. This importance of amino
acids was realized soon after the development of the first cell lines, and a solution of a …

Controlling glycosylation of recombinant proteins produced by CHO cells is highly desired
as it can be directed towards maintaining or increasing product quality. To further our …

Glycosylation is a key critical quality attribute for monoclonal antibodies and other
recombinant proteins because of its impact on effector mechanisms and half‐life. In this …

Here we demonstrate that it is possible to predict and control N‐glycan processing of a
secreted recombinant monoclonal antibody during manufacturing process development …