Flow cytometry: basic principles and applications

A Adan, G Alizada, Y Kiraz, Y Baran… - Critical reviews in …, 2017 - Taylor & Francis
Flow cytometry is a sophisticated instrument measuring multiple physical characteristics of a
single cell such as size and granularity simultaneously as the cell flows in suspension …

Single-cell microfluidic impedance cytometry: From raw signals to cell phenotypes using data analytics

C Honrado, P Bisegna, NS Swami, F Caselli - Lab on a Chip, 2021 - pubs.rsc.org
The biophysical analysis of single-cells by microfluidic impedance cytometry is emerging as
a label-free and high-throughput means to stratify the heterogeneity of cellular systems …

Point-of-care testing (POCT) diagnostic systems using microfluidic lab-on-a-chip technologies

W Jung, J Han, JW Choi, CH Ahn - Microelectronic Engineering, 2015 - Elsevier
Point-of-care testing (POCT) is necessary to provide a rapid diagnostic result for a prompt on-
site diagnosis and treatment. A quick analysis time and high sensitivity, with a sample-to …

Massive image-based single-cell profiling reveals high levels of circulating platelet aggregates in patients with COVID-19

M Nishikawa, H Kanno, Y Zhou, TH Xiao… - Nature …, 2021 - nature.com
A characteristic clinical feature of COVID-19 is the frequent incidence of microvascular
thrombosis. In fact, COVID-19 autopsy reports have shown widespread thrombotic …

Microfluidic approaches for cancer cell detection, characterization, and separation

J Chen, J Li, Y Sun - Lab on a Chip, 2012 - pubs.rsc.org
This article reviews the recent developments in microfluidic technologies for in vitro cancer
diagnosis. We summarize the working principles and experimental results of key microfluidic …

Recent advances in microfluidic techniques for single-cell biophysical characterization

Y Zheng, J Nguyen, Y Wei, Y Sun - Lab on a Chip, 2013 - pubs.rsc.org
Biophysical (mechanical and electrical) properties of living cells have been proven to play
important roles in the regulation of various biological activities at the molecular and cellular …

Positional dependence of particles and cells in microfluidic electrical impedance flow cytometry: Origin, challenges and opportunities

H Daguerre, M Solsona, J Cottet, M Gauthier… - Lab on a Chip, 2020 - pubs.rsc.org
Microfluidic electrical impedance flow cytometry is now a well-known and established
method for single-cell analysis. Given the richness of the information provided by impedance …

Automated biophysical classification of apoptotic pancreatic cancer cell subpopulations by using machine learning approaches with impedance cytometry

C Honrado, A Salahi, SJ Adair, JH Moore, TW Bauer… - Lab on a Chip, 2022 - pubs.rsc.org
Unrestricted cell death can lead to an immunosuppressive tumor microenvironment, with
dysregulated apoptotic signaling that causes resistance of pancreatic cancer cells to …

High-speed single-cell dielectric spectroscopy

D Spencer, H Morgan - ACS sensors, 2020 - ACS Publications
Single-cell impedance cytometry is a label-free analysis technique that is now widely used
to measure the electrical properties of a cell and to differentiate different subpopulations …

The intersection of flow cytometry with microfluidics and microfabrication

ME Piyasena, SW Graves - Lab on a Chip, 2014 - pubs.rsc.org
A modern flow cytometer can analyze and sort particles on a one by one basis at rates of 50
000 particles per second. Flow cytometers can also measure as many as 17 channels of …