Over the past decade, CRISPR has become as much a verb as it is an acronym,
transforming biomedical research and providing entirely new approaches for dissecting all …

Arginase activity and arginine metabolism in immune cells have important consequences for
health and disease. Their dysregulation is commonly observed in cancer, autoimmune …

Cellular differentiation requires extensive alterations in chromatin structure and function,
which is elicited by the coordinated action of chromatin and transcription factors. By contrast …

Genome editing, specifically CRISPR/Cas9 technology, has revolutionized biomedical
research and offers potential cures for genetic diseases. Despite rapid progress, low …

Annotation of immunologic gene function in vivo typically requires the generation of
knockout mice, which is time consuming and low throughput. We previously developed …

Human antigen R (HuR) is a ubiquitously expressed RNA-binding protein, which functions
as an RNA regulator. Overexpression of HuR correlates with high grade tumours and poor …

Abstract The Ataxia telangiectasia and Rad3-related (ATR) inhibitor ceralasertib in
combination with the PD-L1 antibody durvalumab demonstrated encouraging clinical benefit …

There remains a critical need for the precise control of CRISPR (clustered regularly
interspaced short palindromic repeats)-based technologies. Here, we engineer a set of …

Genetically engineered mouse models (GEMMs) transformed the study of organismal
disease phenotypes but are limited by their lengthy generation in embryonic stem cells …

Mice are a widely used pre-clinical model system in large part due to their potential for
genetic manipulation. The ability to manipulate gene expression in specific cells under …