Bacteriophage-based advanced bacterial detection: Concept, mechanisms, and applications

W Hussain, MW Ullah, U Farooq, A Aziz… - Biosensors and …, 2021 - Elsevier
Some bacterial species are deadly disease-causing pathogens with high morbidity and
mortality in humans worldwide. Key interfaces in the transmission of bacterial pathogens …

Integrating viral metagenomics into an ecological framework

P Sommers, A Chatterjee, A Varsani… - Annual review of …, 2021 - annualreviews.org
Viral metagenomics has expanded our knowledge of the ecology of uncultured viruses,
within both environmental (eg, terrestrial and aquatic) and host-associated (eg, plants and …

Species-and site-specific genome editing in complex bacterial communities

BE Rubin, S Diamond, BF Cress, A Crits-Christoph… - Nature …, 2022 - nature.com
Understanding microbial gene functions relies on the application of experimental genetics in
cultured microorganisms. However, the vast majority of bacteria and archaea remain …

High-throughput mapping of the phage resistance landscape in E. coli

VK Mutalik, BA Adler, HS Rishi, D Piya, C Zhong… - PLoS …, 2020 - journals.plos.org
Bacteriophages (phages) are critical players in the dynamics and function of microbial
communities and drive processes as diverse as global biogeochemical cycles and human …

Systematic discovery of pseudomonad genetic factors involved in sensitivity to tailocins

S Carim, AL Azadeh, AE Kazakov, MN Price… - The ISME …, 2021 - academic.oup.com
Tailocins are bactericidal protein complexes produced by a wide variety of bacteria that kill
closely related strains and may play a role in microbial community structure. Thanks to their …

Targeted genome editing of bacteria within microbial communities

BE Rubin, S Diamond, BF Cress, A Crits-Christoph… - BioRxiv, 2020 - biorxiv.org
Knowledge of microbial gene functions comes from manipulating the DNA of individual
species in isolation from their natural communities. While this approach to microbial genetics …

[PDF][PDF] Functional screens of barcoded expression libraries uncover new gene functions in carbon utilization among gut Bacteroidales

YY Huang, MN Price, A Hung, O Gal-Oz, D Ho… - BioRxiv, 2022 - biorxiv.org
A mechanistic understanding of host-microbe interactions in the gut microbiome is hindered
by poorly annotated bacterial genomes. While functional genomics can generate large gene …

Identification of Receptor Binding Proteins in Flagellotropic Agrobacterium Phage 7-7-1

F Gonzalez, BE Scharf - Viruses, 2021 - mdpi.com
The rapid discovery of new and diverse bacteriophages has driven the innovation of
approaches aimed at detailing interactions with their bacterial hosts. Previous studies on …

Deciphering microbial metal toxicity responses via random bar code transposon site sequencing and activity-based metabolomics

MP Thorgersen, J Xue, ELW Majumder… - Applied and …, 2021 - Am Soc Microbiol
To uncover metal toxicity targets and defense mechanisms of the facultative anaerobe
Pantoea sp. strain MT58 (MT58), we used a multiomic strategy combining two global …

Barcoded overexpression screens in gut Bacteroidales identify genes with roles in carbon utilization and stress resistance

YY Huang, MN Price, A Hung, O Gal-Oz… - Nature …, 2024 - nature.com
A mechanistic understanding of host-microbe interactions in the gut microbiome is hindered
by poorly annotated bacterial genomes. While functional genomics can generate large gene …