Fluorescence lifetime measurements and biological imaging

MY Berezin, S Achilefu - Chemical reviews, 2010 - ACS Publications
When a molecule absorbs a photon of appropriate energy, a chain of photophysical events
ensues, such as internal conversion or vibrational relaxation (loss of energy in the absence …

Fluorescence microscopy

JW Lichtman, JA Conchello - Nature methods, 2005 - nature.com
Although fluorescence microscopy permeates all of cell and molecular biology, most
biologists have little experience with the underlying photophysical phenomena …

Fluorescence lifetime imaging (FLIM): Basic concepts and some recent developments

K Suhling, LM Hirvonen, JA Levitt, PH Chung… - Medical photonics, 2015 - Elsevier
Fluorescence lifetime imaging (FLIM) is a key fluorescence microscopy technique to map the
environment and interaction of fluorescent probes. It can report on photophysical events that …

Fluorescence imaging in vivo: recent advances

J Rao, A Dragulescu-Andrasi, H Yao - Current opinion in biotechnology, 2007 - Elsevier
In vivo fluorescence imaging uses a sensitive camera to detect fluorescence emission from
fluorophores in whole-body living small animals. To overcome the photon attenuation in …

One‐pot synthesis of near‐infrared fluorescent gold clusters for cellular fluorescence lifetime imaging

L Shang, N Azadfar, F Stockmar, W Send, V Trouillet… - small, 2011 - Wiley Online Library
A facile strategy to synthesize water‐soluble fluorescent gold nanoclusters (Au NCs)
stabilized with the bidentate ligand dihydrolipoic acid (DHLA) is reported. The DHLA …

Time-resolved fluorescence microscopy

K Suhling, PMW French, D Phillips - Photochemical & Photobiological …, 2005 - pubs.rsc.org
In fluorescence microscopy, the fluorescence emission can be characterised not only by
intensity and position, but also by lifetime, polarization and wavelength. Fluorescence …

[HTML][HTML] Compressed sensing in fluorescence microscopy

G Calisesi, A Ghezzi, D Ancora, C D'Andrea… - Progress in Biophysics …, 2022 - Elsevier
Compressed sensing (CS) is a signal processing approach that solves ill-posed inverse
problems, from under-sampled data with respect to the Nyquist criterium. CS exploits …

Molecular probes for fluorescence lifetime imaging

P Sarder, D Maji, S Achilefu - Bioconjugate chemistry, 2015 - ACS Publications
Visualization of biological processes and pathologic conditions at the cellular and tissue
levels largely relies on the use of fluorescence intensity signals from fluorophores or their …

A 100 fps, time-correlated single-photon-counting-based fluorescence-lifetime imager in 130 nm CMOS

RM Field, S Realov, KL Shepard - IEEE Journal of Solid-State …, 2014 - ieeexplore.ieee.org
A fully-integrated single-photon avalanche diode (SPAD) and time-to-digital converter (TDC)
array for high-speed fluorescence lifetime imaging microscopy (FLIM) in standard 130 nm …

Multi‐wavelength fluorescence lifetime spectroscopy: a new approach to the study of endogenous fluorescence in living cells and tissues

D Chorvat Jr, A Chorvatova - Laser Physics Letters, 2009 - Wiley Online Library
The study of biological systems in their real environmental conditions is crucial to decipher
the true image of structures and processes underlying their functionality. In this regard …