DNA transposition at work
AB Hickman, F Dyda - Chemical reviews, 2016 - ACS Publications
DNA transposons are defined segments of DNA that are able to move from one genomic
location to another. Movement is facilitated by one or more proteins, called the transposase …
location to another. Movement is facilitated by one or more proteins, called the transposase …
Targeted transposition with Tn7 elements: safe sites, mobile plasmids, CRISPR/Cas and beyond
JE Peters - Molecular microbiology, 2019 - Wiley Online Library
Transposon Tn7 is notable for the control it exercises over where transposition events are
directed. One Tn7 integration pathways recognizes a highly conserved attachment (att) site …
directed. One Tn7 integration pathways recognizes a highly conserved attachment (att) site …
Recruitment of CRISPR-Cas systems by Tn7-like transposons
JE Peters, KS Makarova… - Proceedings of the …, 2017 - National Acad Sciences
A survey of bacterial and archaeal genomes shows that many Tn7-like transposons contain
minimal type IF CRISPR-Cas systems that consist of fused cas8f and cas5f, cas7f, and cas6f …
minimal type IF CRISPR-Cas systems that consist of fused cas8f and cas5f, cas7f, and cas6f …
The defense island repertoire of the Escherichia coli pan-genome
It has become clear in recent years that anti-phage defense systems cluster non-randomly
within bacterial genomes in so-called “defense islands”. Despite serving as a valuable tool …
within bacterial genomes in so-called “defense islands”. Despite serving as a valuable tool …
Discovery and characterization of novel type ID CRISPR-guided transposons identified among diverse Tn7-like elements in cyanobacteria
CRISPR-Cas defense systems have been naturally coopted for guide RNA-directed
transposition by Tn7 family bacterial transposons. We find cyanobacterial genomes are rich …
transposition by Tn7 family bacterial transposons. We find cyanobacterial genomes are rich …
Evolutionary and mechanistic diversity of Type IF CRISPR-associated transposons
Canonical CRISPR-Cas systems utilize RNA-guided nucleases for targeted cleavage of
foreign nucleic acids, whereas some nuclease-deficient CRISPR-Cas complexes have been …
foreign nucleic acids, whereas some nuclease-deficient CRISPR-Cas complexes have been …
High Frequency and Diversity of Antimicrobial Activities Produced by Nasal Staphylococcus Strains against Bacterial Competitors
D Janek, A Zipperer, A Kulik, B Krismer… - PLoS …, 2016 - journals.plos.org
The human nasal microbiota is highly variable and dynamic often enclosing major
pathogens such as Staphylococcus aureus. The potential roles of bacteriocins or other …
pathogens such as Staphylococcus aureus. The potential roles of bacteriocins or other …
Novel molecular requirements for CRISPR RNA-guided transposition
CRISPR-associated transposases (CASTs) direct DNA integration downstream of target
sites using the RNA-guided DNA binding activity of nuclease-deficient CRISPR-Cas …
sites using the RNA-guided DNA binding activity of nuclease-deficient CRISPR-Cas …
Cargo Genes of Tn7-Like Transposons Comprise an Enormous Diversity of Defense Systems, Mobile Genetic Elements, and Antibiotic Resistance Genes
Transposition is a major mechanism of horizontal gene mobility in prokaryotes. However,
exploration of the genes mobilized by transposons (cargo) is hampered by the difficulty in …
exploration of the genes mobilized by transposons (cargo) is hampered by the difficulty in …
Modularity and diversity of target selectors in Tn7 transposons
To spread, transposons must integrate into target sites without disruption of essential genes
while avoiding host defense systems. Tn7-like transposons employ multiple mechanisms for …
while avoiding host defense systems. Tn7-like transposons employ multiple mechanisms for …