Lateral flow test engineering and lessons learned from COVID-19

J Budd, BS Miller, NE Weckman, D Cherkaoui… - Nature Reviews …, 2023 - nature.com
The acceptability and feasibility of large-scale testing with lateral flow tests (LFTs) for clinical
and public health purposes has been demonstrated during the COVID-19 pandemic. LFTs …

CRISPR-Based diagnostics: challenges and potential solutions toward point-of-care applications

A Ghouneimy, A Mahas, T Marsic, R Aman… - ACS Synthetic …, 2022 - ACS Publications
The COVID-19 pandemic has challenged the conventional diagnostic field and revealed the
need for decentralized Point of Care (POC) solutions. Although nucleic acid testing is …

Nanoplasmonic amplification in microfluidics enables accelerated colorimetric quantification of nucleic acid biomarkers from pathogens

T AbdElFatah, M Jalali, SG Yedire, I I. Hosseini… - Nature …, 2023 - nature.com
Deployment of nucleic acid amplification assays for diagnosing pathogens in point-of-care
settings is a challenge due to lengthy preparatory steps. We present a molecular diagnostic …

Glow-in-the-dark infectious disease diagnostics using Crispr-Cas9-based Split luciferase complementation

HJ van der Veer, EA van Aalen… - ACS Central …, 2023 - ACS Publications
Nucleic acid detection methods based on CRISPR and isothermal amplification techniques
show great potential for point-of-care diagnostic applications. However, most current …

CRISPR-based biosensors for pathogenic biosafety

H Yang, R Ledesma-Amaro, H Gao, Y Ren… - Biosensors and …, 2023 - Elsevier
Pathogenic biosafety is a worldwide concern. Tools for analyzing pathogenic biosafety, that
are precise, rapid and field-deployable, are highly demanded. Recently developed …

A programmable DNAzyme for the sensitive detection of nucleic acids

C Shi, D Yang, X Ma, L Pan, Y Shao… - Angewandte Chemie …, 2024 - Wiley Online Library
Nucleic acids in biofluids are emerging biomarkers for the molecular diagnostics of
diseases, but their clinical use has been hindered by the lack of sensitive detection assays …

CRISPR‐Mediated Profiling of Viral RNA at Single‐Nucleotide Resolution

D Chen, W Huang, Y Zhang, B Chen… - Angewandte Chemie …, 2023 - Wiley Online Library
Mass pathogen screening is critical to preventing the outbreaks and spread of infectious
diseases. The large‐scale epidemic of COVID‐19 and the rapid mutation of the severe acute …

New design strategies for ultra-specific CRISPR-Cas13a-based RNA detection with single-nucleotide mismatch sensitivity

AM Molina Vargas, S Sinha, R Osborn… - Nucleic acids …, 2024 - academic.oup.com
An increasingly pressing need for clinical diagnostics has required the development of novel
nucleic acid-based detection technologies that are sensitive, fast, and inexpensive, and that …

Next-generation CRISPR-based diagnostic tools for human diseases

T Wang, Z Wang, L Bai, X Zhang, J Feng, C Qian… - TrAC Trends in …, 2023 - Elsevier
Accurate diagnosis of human diseases is crucial for effective prevention, clinical treatment,
and prognosis. The World Health Organization (WHO) has provided guidelines for molecular …

CRISPR-based biosensing strategies: technical development and application prospects

T Tian, X Zhou - Annual Review of Analytical Chemistry, 2023 - annualreviews.org
Biosensing based on CRISPR-Cas systems is a young but rapidly evolving technology. The
unprecedented properties of the CRISPR-Cas system provide an innovative tool for …