Piecing together a ciliome

PN Inglis, KA Boroevich, MR Leroux - TRENDS in Genetics, 2006 - cell.com
Cilia are slender microtubule-based appendages that emanate from the surfaces of a large
proportion of eukaryotic cells. The motile and non-motile forms of cilia represent bona fide …

Cilia distal domain: Diversity in evolutionarily conserved structures

H Soares, B Carmona, S Nolasco, L Viseu Melo… - Cells, 2019 - mdpi.com
Eukaryotic cilia are microtubule-based organelles that protrude from the cell surface to fulfill
sensory and motility functions. Their basic structure consists of an axoneme templated by a …

Proteomic analysis of isolated chlamydomonas centrioles reveals orthologs of ciliary-disease genes

LC Keller, EP Romijn, I Zamora, JR Yates, WF Marshall - Current Biology, 2005 - cell.com
Background: The centriole is one of the most enigmatic organelles in the cell. Centrioles are
cylindrical, microtubule-based barrels found in the core of the centrosome. Centrioles also …

Genome-wide transcriptional analysis of flagellar regeneration in Chlamydomonas reinhardtii identifies orthologs of ciliary disease genes

V Stolc, MP Samanta, W Tongprasit… - Proceedings of the …, 2005 - National Acad Sciences
The important role that cilia and flagella play in human disease creates an urgent need to
identify genes involved in ciliary assembly and function. The strong and specific induction of …

Mutations in DNAJB13, encoding an HSP40 family member, cause primary ciliary dyskinesia and male infertility

E El Khouri, L Thomas, L Jeanson, E Bequignon… - The American Journal of …, 2016 - cell.com
Primary ciliary dyskinesia (PCD) is an autosomal-recessive disease due to functional or ultra-
structural defects of motile cilia. Affected individuals display recurrent respiratory-tract …

Ciliary tubulin and its post-translational modifications

J Gaertig, D Wloga - Current topics in developmental biology, 2008 - Elsevier
Tubulin, the most abundant axonemal protein, is extensively modified by several highly
conserved post-translational mechanisms including acetylation, detyrosination …

[HTML][HTML] Tubulin cofactor A gene silencing in mammalian cells induces changes in microtubule cytoskeleton, cell cycle arrest and cell death

S Nolasco, J Bellido, J Gonçalves, JC Zabala… - FEBS letters, 2005 - Elsevier
Microtubules are polymers of α/β-tubulin participating in essential cell functions. A multistep
process involving distinct molecular chaperones and cofactors produces new tubulin …

RPGRIP1L helps to establish the ciliary gate for entry of proteins

H Lin, S Guo, SK Dutcher - Journal of cell science, 2018 - journals.biologists.com
Mutations in transition zone genes change the composition of the ciliary proteome. We
isolated new mutations in RPGRIP1L (denotated as RPG1 in algae) that affect the …

Dimeric heat shock protein 40 binds radial spokes for generating coupled power strokes and recovery strokes of 9+ 2 flagella

C Yang, HA Owen, P Yang - The Journal of Cell Biology, 2008 - rupress.org
T-shape radial spokes regulate flagellar beating. However, the precise function and
molecular mechanism of these spokes remain unclear. Interestingly, Chlamydomonas …

Label-free proteomic comparison reveals ciliary and nonciliary phenotypes of IFT-A mutants

JC Leggere, JVK Hibbard, O Papoulas… - Molecular Biology of …, 2024 - Am Soc Cell Biol
DIFFRAC is a powerful method for systematically comparing proteome content and
organization between samples in a high-throughput manner. By subjecting control and …