Protein purification and analysis: next generation Western blotting techniques

M Mishra, S Tiwari, AV Gomes - Expert review of proteomics, 2017 - Taylor & Francis
Introduction: Western blotting is one of the most commonly used techniques in molecular
biology and proteomics. Since western blotting is a multistep protocol, variations and errors …

[HTML][HTML] Protein immobilization techniques for microfluidic assays

D Kim, AE Herr - Biomicrofluidics, 2013 - pubs.aip.org
Microfluidic systems have shown unequivocal performance improvements over conventional
bench-top assays across a range of performance metrics. For example, specific advances …

Micro total analysis systems for cell biology and biochemical assays

ML Kovarik, PC Gach, DM Ornoff, Y Wang… - Analytical …, 2012 - ACS Publications
Novel applications of micro total analysis systems (μTAS) are addressing fundamental
biological questions, creating new biomedical reagents, and developing innovative cell and …

[图书][B] Making and using antibodies: a practical handbook

GC Howard, MR Kaser - 2013 - books.google.com
Antibodies protect us from a wide range of infectious diseases and cancers and have
become an indispensable tool in science—both for conventional immune response research …

Ultrasensitive microfluidic solid-phase ELISA using an actuatable microwell-patterned PDMS chip

T Wang, M Zhang, DD Dreher, Y Zeng - Lab on a Chip, 2013 - pubs.rsc.org
Quantitative detection of low abundance proteins is of significant interest for biological and
clinical applications. Here we report an integrated microfluidic solid-phase ELISA platform …

Automated microfluidic protein immunoblotting

M He, AE Herr - Nature protocols, 2010 - nature.com
This protocol describes regional photopatterning of polyacrylamide gels in glass microfluidic
devices as a platform for seamless integration of multiple assay steps. The technology …

Multiplexed western blotting using microchip electrophoresis

S Jin, MD Furtaw, H Chen, DT Lamb… - Analytical …, 2016 - ACS Publications
Western blotting is a commonly used protein assay that combines the selectivity of
electrophoretic separation and immunoassay. The technique is limited by long time, manual …

Emerging techniques of western blotting for purification and analysis of protein

KK Singh, A Gupta, C Bharti, H Sharma - Future Journal of Pharmaceutical …, 2021 - Springer
Background Western blotting is frequently employed in molecular techniques like
Proteomics and Biology. Because it is a sequential framework, differences and inaccuracies …

Western blotting using capillary electrophoresis

GJ Anderson, C M. Cipolla, RT Kennedy - Analytical chemistry, 2011 - ACS Publications
A microscale Western blotting system based on separating sodium-dodecyl sulfate protein
complexes by capillary gel electrophoresis followed by deposition onto a blotting membrane …

The development of microfluidic-based western blotting: Technical advances and future perspectives

CT Desire, RD Arrua, XL Strudwick, Z Kopecki… - … of Chromatography A, 2023 - Elsevier
Over the past two decades significant technical advancement in the field of western blotting
has been made possible through the utilization of microfluidic technologies. In this review …