Off-target effects in CRISPR/Cas9 gene editing
C Guo, X Ma, F Gao, Y Guo - Frontiers in bioengineering and …, 2023 - frontiersin.org
Gene editing stands for the methods to precisely make changes to a specific nucleic acid
sequence. With the recent development of the clustered regularly interspaced short …
sequence. With the recent development of the clustered regularly interspaced short …
Assessing and advancing the safety of CRISPR-Cas tools: from DNA to RNA editing
CRISPR-Cas gene editing has revolutionized experimental molecular biology over the past
decade and holds great promise for the treatment of human genetic diseases. Here we …
decade and holds great promise for the treatment of human genetic diseases. Here we …
Genome editing with CRISPR–Cas nucleases, base editors, transposases and prime editors
The development of new CRISPR–Cas genome editing tools continues to drive major
advances in the life sciences. Four classes of CRISPR–Cas-derived genome editing agents …
advances in the life sciences. Four classes of CRISPR–Cas-derived genome editing agents …
Latest developed strategies to minimize the off-target effects in CRISPR-Cas-mediated genome editing
Gene editing that makes target gene modification in the genome by deletion or addition has
revolutionized the era of biomedicine. Clustered regularly interspaced short palindromic …
revolutionized the era of biomedicine. Clustered regularly interspaced short palindromic …
Neuronal DNA double-strand breaks lead to genome structural variations and 3D genome disruption in neurodegeneration
Persistent DNA double-strand breaks (DSBs) in neurons are an early pathological hallmark
of neurodegenerative diseases including Alzheimer's disease (AD), with the potential to …
of neurodegenerative diseases including Alzheimer's disease (AD), with the potential to …
Engineered CRISPR–Cas12a variants with increased activities and improved targeting ranges for gene, epigenetic and base editing
Broad use of CRISPR–Cas12a (formerly Cpf1) nucleases has been hindered by the
requirement for an extended TTTV protospacer adjacent motif (PAM). To address this …
requirement for an extended TTTV protospacer adjacent motif (PAM). To address this …
CRISPR-based therapeutic genome editing: strategies and in vivo delivery by AAV vectors
The development of clustered regularly interspaced short-palindromic repeat (CRISPR)-
based biotechnologies has revolutionized the life sciences and introduced new therapeutic …
based biotechnologies has revolutionized the life sciences and introduced new therapeutic …
Base editing with a Cpf1–cytidine deaminase fusion
X Li, Y Wang, Y Liu, B Yang, X Wang, J Wei, Z Lu… - Nature …, 2018 - nature.com
The targeting range of CRISPR–Cas9 base editors (BEs) is limited by their G/C-rich
protospacer-adjacent motif (PAM) sequences. To overcome this limitation, we developed a …
protospacer-adjacent motif (PAM) sequences. To overcome this limitation, we developed a …
Unbiased detection of CRISPR off-targets in vivo using DISCOVER-Seq
CRISPR-Cas genome editing induces targeted DNA damage but can also affect off-target
sites. Current off-target discovery methods work using purified DNA or specific cellular …
sites. Current off-target discovery methods work using purified DNA or specific cellular …
Increasing the specificity of CRISPR systems with engineered RNA secondary structures
CRISPR (clustered regularly interspaced short palindromic repeat) systems have been
broadly adopted for basic science, biotechnology, and gene and cell therapy. In some …
broadly adopted for basic science, biotechnology, and gene and cell therapy. In some …