Clavibacter nebraskensis causing Goss's wilt of maize: Five decades of detaining the enemy in the New World

E Osdaghi, AE Robertson… - Molecular Plant …, 2023 - Wiley Online Library
Goss's bacterial wilt and leaf blight of maize (Zea mays) caused by the gram‐positive
coryneform bacterium Clavibacter nebraskensis is an economically important disease in …

Multiplex recombinase polymerase amplification assay developed using unique genomic regions for rapid on-site detection of genus Clavibacter and C. nebraskensis

A Larrea-Sarmiento, JP Stack, AM Alvarez, M Arif - Scientific reports, 2021 - nature.com
Clavibacter is an agriculturally important bacterial genus comprising nine host-specific
species/subspecies including C. nebraskensis (Cn), which causes Goss's wilt and blight of …

Three new species, Xanthomonas hawaiiensis sp. nov., Stenotrophomonas aracearum sp. nov., and Stenotrophomonas oahuensis sp. nov., isolated from the …

SC Chuang, S Dobhal, AM Alvarez, M Arif - Frontiers in Microbiology, 2024 - frontiersin.org
Xanthomonas and Stenotrophomonas are closely related genera in the family
Lysobacteraceae. In our previous study of aroid-associated bacterial strains, most strains …

Terminology and guidelines for diagnostic assay development and validation: Best practices for molecular tests

D Groth-Helms, Y Rivera, FN Martin, M Arif… - …, 2023 - Am Phytopath Society
Effective use of diagnostic assays is essential for the early detection of plant pathogens and
mitigation of potential disease impacts. Assay developers require a full understanding of the …

Comparative genomics reveals signature regions used to develop a robust and sensitive multiplex TaqMan real‐time qPCR assay to detect the genus Dickeya and …

S Dobhal, G Boluk, B Babler, MJ Stulberg… - Journal of Applied …, 2020 - academic.oup.com
Aims Dickeya species are high consequence plant pathogenic bacteria; associated with
potato disease outbreaks and subsequent economic losses worldwide. Early, accurate and …

New Assays for Rapid Detection of Beet Leafhopper-Associated Plant Pathogens, 'Candidatus Phytoplasma trifolii', Beet Curly Top Virus, and Spiroplasma citri

KD Swisher Grimm, CJ Gorman, JM Crosslin - Plant Disease, 2023 - Am Phytopath Society
The beet leafhopper Circulifer tenellus is an important pest of agricultural crops in the United
States, where it transmits beet curly top virus, beet leafhopper-transmitted virescence agent …

Multiple internal controls enhance reliability for PCR and real time PCR detection of Rathayibacter toxicus

M Arif, GY Busot, R Mann, B Rodoni, JP Stack - Scientific Reports, 2021 - nature.com
Rathayibacter toxicus is a toxigenic bacterial plant pathogen indigenous to Australia and
South Africa. A threat to livestock industries globally, the bacterium was designated a US …

Field-deployable recombinase polymerase amplification assay for specific, sensitive and rapid detection of the US select agent and toxigenic bacterium, Rathayibacter …

M Arif, GY Busot, R Mann, B Rodoni, JP Stack - Biology, 2021 - mdpi.com
Simple Summary Early, accurate, and rapid detection of R. toxicus is extremely important to
improve inspections of imported annual ryegrass hay and seed at ports of entry and …

Development of a multiplex TaqMan qPCR targeting unique genomic regions for the specific and sensitive detection of Pectobacterium species and P. parmentieri

D Arizala, S Dobhal, B Babler… - Journal of Applied …, 2022 - academic.oup.com
Aim The newly defined species Pectobacterium parmentieri has emerged as an aggressive
pathogen that causes soft rot and blackleg diseases on potato and has been widely …

Improved multiplex TaqMan qPCR assay with universal internal control offers reliable and accurate detection of Clavibacter michiganensis

S Ramachandran, S Dobhal… - Journal of applied …, 2021 - academic.oup.com
Abstract Aim Clavibacter michiganensis (Cm) is a seed‐borne plant pathogen that
significantly reduces tomato production worldwide. Due to repeated outbreaks and rapid …