Eukaryotic mRNA with its 5′-cap is of central importance for the cell. Many studies involving
mRNA require reliable preparation and modification of 5′-capped RNAs. Depending on the …

RNA modifications immensely expand the diversity of the transcriptome, thereby influencing
the function, localization, and stability of RNA. One prominent example of an RNA …

RNA capping and decapping are thought to be distinctive features of eukaryotes. The redox
cofactor NAD was recently discovered to be attached to small regulatory RNAs in bacteria in …

Here we describe a protocol for NAD captureSeq that allows for the identification of
nicotinamide-adenine dinucleotide (NAD)-capped RNA sequences in total RNA samples …

In recent years, several noncanonical RNA caps derived from cofactors and metabolites
have been identified. Purine-containing RNA caps have been extensively studied, with …

The cap is a natural modification present at the 5'ends of eukaryotic messenger RNA
(mRNA), which because of its unique structural features, mediates essential biological …

Today, RNA is no longer seen as a mere intermediary, transferring information from genes to
proteins. It has become more and more obvious that RNA plays additional biological roles in …

The mechanisms by which viruses hijack the genetic machinery of the cells they infect are of
current interest. When bacteriophage T4 infects Escherichia coli, it uses three different …

RNA modifications are widely distributed in Nature, and their thorough analysis helps
answering fundamental biological questions. Nowadays, mass spectrometry or deep …

Nicotinamide is an important functional compound and, in the form of nicotinamide adenine
dinucleotide (NAD), is used as a co-factor by protein-based enzymes to catalyze redox …