The distinguishing electrical properties of cancer cells

E Di Gregorio, S Israel, M Staelens, G Tankel… - Physics of life …, 2022 - Elsevier
In recent decades, medical research has been primarily focused on the inherited aspect of
cancers, despite the reality that only 5–10% of tumours discovered are derived from genetic …

Effect of experimental electrical and biological parameters on gene transfer by electroporation: a systematic review and Meta-analysis

T Potočnik, A Maček Lebar, Š Kos, M Reberšek, E Pirc… - Pharmaceutics, 2022 - mdpi.com
The exact mechanisms of nucleic acid (NA) delivery with gene electrotransfer (GET) are still
unknown, which represents a limitation for its broader use. Further, not knowing the effects …

Calcium electroporation causes ATP depletion in cells and is effective both in microsecond and nanosecond pulse range as a modality of electrochemotherapy

Calcium electroporation is a modality of electrochemotherapy (ECT), which is based on
intracellular electric field-mediated delivery of cytotoxic doses of calcium into the cells …

Efficient gene transfection by electroporation—in vitro and in silico study of pulse parameters

T Potočnik, S Sachdev, T Polajžer, A Maček Lebar… - Applied Sciences, 2022 - mdpi.com
Gene electrotransfer (GET) is a widely used method for nucleic acids' delivery into cells. We
explored, evaluated, and demonstrated the potential use of different pulse durations for …

Calcium Electrochemotherapy for Tumor Eradication and the Potential of High-Frequency Nanosecond Protocols

E Radzevičiūtė-Valčiukė, A Želvys, E Mickevičiūtė… - Pharmaceuticals, 2023 - mdpi.com
Calcium electroporation (CaEP) is an innovative approach to treating cancer, involving the
internalization of supraphysiological amounts of calcium through electroporation, which …

Negative effects of cancellation during nanosecond range High-Frequency calcium based electrochemotherapy in vitro

N Rembiałkowska, W Szlasa… - International Journal of …, 2023 - Elsevier
Drug delivery using nanosecond pulsed electric fields is a new branch of electroporation-
based treatments, which potentially can substitute European standard operating procedures …

Effects of buffer composition and plasmid toxicity on electroporation-based non-viral gene delivery in mammalian cells using bursts of nanosecond and microsecond …

E Radzevičiūtė-Valčiukė, J Gečaitė… - … in Bioengineering and …, 2024 - frontiersin.org
Gene electrotransfer (GET) is non-viral gene delivery technique, also known as
electroporation-mediated gene delivery or electrotransfection. GET is a method used to …

Mitochondrial depolarization and ATP loss during high frequency nanosecond and microsecond electroporation

P Malakauskaitė, A Želvys, A Zinkevičienė… - …, 2024 - Elsevier
It is predicted that ultra-short electric field pulses (nanosecond) can selectively permeabilize
intracellular structures (eg, mitochondria) without significant effects on the outer cell plasma …

[HTML][HTML] Extracellular vesicle loaded with miR-494-3p can suppress ovarian cancer cell

S Wang, B Pan, J Zhu, P Wang, K Li - Journal of Drug Delivery Science and …, 2024 - Elsevier
Objective Ovarian cancer (OC) accounts for the highest mortality rate among all
gynecological malignancies. We intend to develop engineered exosomes to deliver the …

[HTML][HTML] Expression of GFP and DsRed fluorescent proteins after gene electrotransfer of tumour cells in vitro

T Komel, M Bosnjak, G Sersa, M Cemazar - Bioelectrochemistry, 2023 - Elsevier
Fluorescent reporter genes are widely used to study the transfection of various types of
primary cells and cell lines. The aim of our research was to investigate the expression …