The impact of cultivation strategy on the cost of recombinant protein production is crucial for
defining cost-effective bioreactor operation conditions. This paper presents a methodology …

Background Recombinant protein production can be stressful to the host organism. The
extent of stress is determined by the specific properties of the recombinant transcript and …

Background Glycoconjugated vaccines composed of polysaccharide antigens covalently
linked to immunogenic carrier proteins have proved to belong to the most effective and …

Auto‐induction media containing glucose, lactose, and glycerol are a simple and efficient
approach for high‐throughput protein expression in Escherichia coli with lac‐derived …

Several studies have searched for new antigens to produce pneumococcal vaccines that are
more effective and could provide broader coverage, given the great number of serotypes …

Gene encoding glutaminase-free l-asparaginase II (ans B2) from Pectobacterium
carotovorum MTCC 1428 was cloned into pHT43, transformed in Bacillus subtilis WB800N …

Automation, parallelization and autonomous operation of standard lab equipment, usually
applied for manual bioprocess development, is considered as the key for reduction of …

The impact of recombinant protein production (RPP) on host cells and the metabolic burden
associated with it undermine the efficiency of the production system. This study utilized …

Abstract Background Penicillin G acylase (PGA) is used industrially to catalyze the
hydrolysis of penicillin G to obtain 6-aminopenicillanic acid. In Escherichia coli, the most …

We studied the crucial components which elevate the expression of recombinant novel
glutaminase free L-asparaginase II (rL-asp II) from Bacillus subtilis WB800N. The Plackett …