Designing microbial cell factories for the production of chemicals

JS Cho, GB Kim, H Eun, CW Moon, SY Lee - Jacs Au, 2022 - ACS Publications
The sustainable production of chemicals from renewable, nonedible biomass has emerged
as an essential alternative to address pressing environmental issues arising from our heavy …

Translation in prokaryotes

MV Rodnina - Cold Spring Harbor perspectives in …, 2018 - cshperspectives.cshlp.org
This review summarizes our current understanding of translation in prokaryotes, focusing on
the mechanistic and structural aspects of each phase of translation: initiation, elongation …

High-throughput recombinant protein expression in Escherichia coli: current status and future perspectives

B Jia, CO Jeon - Open biology, 2016 - royalsocietypublishing.org
The ease of genetic manipulation, low cost, rapid growth and number of previous studies
have made Escherichia coli one of the most widely used microorganism species for …

A deep learning approach to programmable RNA switches

NM Angenent-Mari, AS Garruss, LR Soenksen… - Nature …, 2020 - nature.com
Engineered RNA elements are programmable tools capable of detecting small molecules,
proteins, and nucleic acids. Predicting the behavior of these synthetic biology components …

Improved designs for pET expression plasmids increase protein production yield in Escherichia coli

PJ Shilling, K Mirzadeh, AJ Cumming… - Communications …, 2020 - nature.com
The pET series of expression plasmids are widely used for recombinant protein production
in Escherichia coli. The genetic modules controlling transcription and translation in these …

The ribosome in action: Tuning of translational efficiency and protein folding

MV Rodnina - Protein Science, 2016 - Wiley Online Library
Marina V. Rodnina is the winner of the 2015 Hans Neurath Award. Abstract The cellular
proteome is shaped by the combined activities of the gene expression and quality control …

An automated model test system for systematic development and improvement of gene expression models

AC Reis, HM Salis - ACS synthetic biology, 2020 - ACS Publications
Gene expression models greatly accelerate the engineering of synthetic metabolic pathways
and genetic circuits by predicting sequence-function relationships and reducing trial-and …

Precise quantification of translation inhibition by mRNA structures that overlap with the ribosomal footprint in N-terminal coding sequences

A Espah Borujeni, D Cetnar, I Farasat… - Nucleic acids …, 2017 - academic.oup.com
A mRNA's translation rate is controlled by several sequence determinants, including the
presence of RNA structures within the N-terminal regions of its coding sequences. However …

CRISPathBrick: Modular Combinatorial Assembly of Type II-A CRISPR Arrays for dCas9-Mediated Multiplex Transcriptional Repression in E. coli

BF Cress, OD Toparlak, S Guleria… - ACS synthetic …, 2015 - ACS Publications
Programmable control over an addressable global regulator would enable simultaneous
repression of multiple genes and would have tremendous impact on the field of synthetic …

Toolboxes for cyanobacteria: recent advances and future direction

T Sun, S Li, X Song, J Diao, L Chen, W Zhang - Biotechnology advances, 2018 - Elsevier
Photosynthetic cyanobacteria are important primary producers and model organisms for
studying photosynthesis and elements cycling on earth. Due to the ability to absorb sunlight …