The ever-expanding set of CRISPR technologies and their programmable RNA-guided
nucleases exhibit remarkable flexibility in DNA targeting. However, this flexibility comes with …

Human immunodeficiency virus (HIV) infections and HIV-induced acquired
immunodeficiency syndrome (AIDS) continue to represent a global health burden. There is …

CRISPR-Cas systems recognize foreign genetic material using CRISPR RNAs (crRNAs). In
type II systems, a trans-activating crRNA (tracrRNA) hybridizes to crRNAs to drive their …

CRISPR-associated Tn7 transposons (CASTs) co-opt cas genes for RNA-guided
transposition. CASTs are exceedingly rare in genomic databases; recent surveys have …

Because of the outstanding contribution in genome editing, CRISPR has undoubtedly
become the most popular technology around the world and two pioneers are awarded the …

CRISPR (clustered regularly interspaced short palindromic repeats)/Cas9 is a unique
genome editing tool that can be easily used in a wide range of applications, including …

CRISPR technologies increasingly require spatiotemporal and dosage control of nuclease
activity. One promising strategy involves linking nuclease activity to a cell's transcriptional …

Abstract The RNA-guided CRISPR/Cas9 system is a powerful tool for genome editing, but its
targeting scope is limited by the protospacer-adjacent motif (PAM). To expand the target …

Fungal species have the capability of producing an overwhelming diversity of bioactive
substances that can have beneficial but also detrimental effects on human health. These so …

Existing CRISPR/Cas12a‐based diagnostic platforms offer accurate and vigorous
monitoring of nucleic acid targets, but have the potential to be further optimized for more …