Recent advanced biophysical techniques allow us to monitor the cellular dynamics of
biologically important molecules in real time. Bright, stable fluorophores are needed to …

Single-molecule tracking provides direct real-time measurements of protein dynamics in live
cells with high spatiotemporal resolution. In the last decade, this method has been adopted …

Single-molecule tracking (SMT) is a powerful approach to quantify the biophysical
parameters of protein dynamics in live cells. Here, we describe a protocol for SMT in live …

Abstract Single-Molecule Tracking (SMT) is a powerful method to quantify protein dynamics
in live cells. Recently, we have established a data analysis pipeline for estimating various …

Aurora kinase B, Ipl1 in Saccharomyces cerevisiae, is the master regulator of cell division
required for checkpoint regulation, spindle assembly and disassembly, chromosome …

In this dissertation, I investigated the molecular mechanism of clathrin-mediated endocytosis
(CME) in fission yeast with a sparse labeling strategy to track endocytic proteins at the single …

Single-molecule tracking provides direct real-time measurements of protein dynamics in live
cells with high spatiotemporal resolution. In the last decade, this method has been adopted …