It has been 10 years since the introduction of modern transposon-insertion sequencing (TIS)
methods, which combine genome-wide transposon mutagenesis with high-throughput …

Microbiology is at a turning point in its 120-year history. Widespread next-generation
sequencing has revealed genetic complexity among bacteria that could hardly have been …

Essential genes refer to genes that are required by an organism to survive under specific
conditions. Studies of the minimal-gene-set for bacteria have elucidated fundamental …

Tuberculosis (TB) is among the most difficult infections to treat, requiring several months of
multidrug therapy to produce a durable cure. The reasons necessitating long treatment times …

Staphylococcus aureus is a Gram-positive pathogen responsible for antibiotic-resistant
infections. To identify vulnerabilities in cell envelope biogenesis that may overcome …

The ATP synthase is a multicomponent enzyme that is largely conserved across the
kingdoms of life. In many species the ATP synthase is central in the synthesis of ATP by …

Staphylococcus aureus is a pathogen that causes critical diseases, such as pneumonia,
endocarditis, and bacteremia, upon gaining access to the bloodstream of the host. Because …

Antibiotic resistance is a serious global health concern that may have significant social and
financial consequences. Methicillin-resistant Staphylococcus aureus (MRSA) infection is …

Methicillin-resistant Staphylococcus aureus (MRSA) infections are some of the most
common antibiotic-resistant infections, often exacerbated by the formation of biofilms. Here …

Gram-positive bacterial cells are protected from the environment by a cell envelope that is
comprised of a thick layer of peptidoglycan that maintains cell shape and teichoic acid …