[PDF][PDF] Inhibition and facilitation of nucleic acid amplification

IG Wilson - Applied and environmental microbiology, 1997 - Am Soc Microbiol
Factors that inhibit the amplification of nucleic acids by PCR are present with target DNAs
from many sources. The inhibitors generally act at one or more of three essential points in …

Relevance of nucleic acid amplification techniques for diagnosis of respiratory tract infections in the clinical laboratory

M Ieven, H Goossens - Clinical Microbiology Reviews, 1997 - Am Soc Microbiol
Clinical laboratories are increasingly receiving requests to perform nucleic acid amplification
tests for the detection of a wide variety of infectious agents. In this paper, the efficiency of …

Simple and rapid detection of Streptococcus mutans and Streptococcus sobrinus in human saliva by polymerase chain reaction

T Oho, Y Yamashita, Y Shimazaki… - Oral microbiology …, 2000 - Wiley Online Library
Streptococcus mutans and Streptococcus sobrinus are major pathogens causing dental
caries in humans. A simple and rapid method to detect these species in human saliva …

Assessment by meta-analysis of PCR for diagnosis of smear-negative pulmonary tuberculosis

OL Sarmiento, KA Weigle, J Alexander… - Journal of Clinical …, 2003 - Am Soc Microbiol
We conducted a meta-analysis to assess the performance of PCR for the diagnosis of smear-
negative pulmonary tuberculosis (SPT) and to identify factors that account for differences in …

Quantitative PCR Assay To MeasureAspergillus fumigatus Burden in a Murine Model of Disseminated Aspergillosis: Demonstration of Efficacy of Caspofungin …

JC Bowman, GK Abruzzo, JW Anderson… - Antimicrobial agents …, 2001 - Am Soc Microbiol
ABSTRACT Caspofungin acetate (MK-0991) is an antifungal antibiotic that inhibits the
synthesis of 1, 3-β-d-glucan, an essential component of the cell wall of several pathogenic …

Rapid diagnosis of extrapulmonary tuberculosis by PCR: impact of sample preparation and DNA extraction

S Honore-Bouakline, JP Vincensini… - Journal of clinical …, 2003 - Am Soc Microbiol
In cases of suspected extrapulmonary tuberculosis, rapid and accurate laboratory diagnosis
is of prime importance, since traditional techniques of detecting acid-fast bacilli have …

In-house nucleic acid amplification tests for the detection of Mycobacterium tuberculosis in sputum specimens: meta-analysis and meta-regression

LL Flores, M Pai, JM Colford, LW Riley - BMC microbiology, 2005 - Springer
Background More than 200 studies related to nucleic acid amplification (NAA) tests to detect
Mycobacterium tuberculosis directly from clinical specimens have appeared in the world …

Optimization of the PCR for detection of Staphylococcus aureus nuc gene in bovine milk

CH Kim, M Khan, DE Morin, WL Hurley… - Journal of dairy …, 2001 - Elsevier
Staphylococcus aureus is an economically important and a major mastitis-causing pathogen
that also poses food safety and antimicrobial resistance threats. Substances in mastitic milk …

Detection of Porphyromonas gingivalisfrom Saliva by PCR by Using a Simple Sample-Processing Method

J Mättö, M Saarela, S Alaluusua, V Oja… - Journal of Clinical …, 1998 - Am Soc Microbiol
Simple sample-processing methods for PCR detection of Porphyromonas gingivalis, a major
pathogen causing adult periodontitis, from saliva were studied. The ability to detect P …

Genotyping CagA, VacA subtype, IceA1, and BabA of Helicobacter pylori isolates from Korean patients, and their association with gastroduodenal diseases

SY Kim, CW Woo, YM Lee, BR Son… - Journal of Korean …, 2001 - synapse.koreamed.org
The genetic status of cagA, vacA subtype, iceA1, and babA, and the relationship to
gastroduodenal diseases were assessed in Helicobacter pylori isolates in Korea. Seventy …