Protein cages: from fundamentals to advanced applications

TGW Edwardson, MD Levasseur, S Tetter… - Chemical …, 2022 - ACS Publications
Proteins that self-assemble into polyhedral shell-like structures are useful molecular
containers both in nature and in the laboratory. Here we review efforts to repurpose diverse …

Bacterial microcompartments

CA Kerfeld, C Aussignargues, J Zarzycki… - Nature Reviews …, 2018 - nature.com
Bacterial microcompartments (BMCs) are self-assembling organelles that consist of an
enzymatic core that is encapsulated by a selectively permeable protein shell. The potential …

The formate bio-economy

O Yishai, SN Lindner, JG de la Cruz… - Current Opinion in …, 2016 - Elsevier
Highlights•Formate is an ideal mediator between the physicochemical and biological
realms.•Formate can be efficiently produced from various highly available …

[HTML][HTML] Synthetic metabolism: metabolic engineering meets enzyme design

TJ Erb, PR Jones, A Bar-Even - Current opinion in chemical biology, 2017 - Elsevier
Highlights•Multiple levels of metabolic engineering can be defined, from 'copy and paste'to
various forms of 'synthetic metabolism'.•The biochemical solution space for 'synthetic …

Assembly, function and evolution of cyanobacterial carboxysomes

CA Kerfeld, MR Melnicki - Current opinion in plant biology, 2016 - Elsevier
Highlights•Alpha and beta carboxysomes differ in composition, assembly, and
evolution.•Alphas appear to co-assemble shell and cargo proteins; betas assemble inside …

A catalog of the diversity and ubiquity of bacterial microcompartments

M Sutter, MR Melnicki, F Schulz, T Woyke… - Nature …, 2021 - nature.com
Bacterial microcompartments (BMCs) are organelles that segregate segments of metabolic
pathways which are incompatible with surrounding metabolism. BMCs consist of a …

Bacterial microcompartments and the modular construction of microbial metabolism

CA Kerfeld, O Erbilgin - Trends in microbiology, 2015 - cell.com
Bacterial microcompartments (BMCs) are protein-bound organelles predicted to be present
across 23 bacterial phyla. BMCs facilitate carbon fixation as well as the aerobic and …

Bacterial encapsulins as orthogonal compartments for mammalian cell engineering

F Sigmund, C Massner, P Erdmann, A Stelzl… - Nature …, 2018 - nature.com
We genetically controlled compartmentalization in eukaryotic cells by heterologous
expression of bacterial encapsulin shell and cargo proteins to engineer enclosed enzymatic …

Selective molecular transport through the protein shell of a bacterial microcompartment organelle

C Chowdhury, S Chun, A Pang… - Proceedings of the …, 2015 - National Acad Sciences
Bacterial microcompartments are widespread prokaryotic organelles that have important
and diverse roles ranging from carbon fixation to enteric pathogenesis. Current models for …

Programmed loading and rapid purification of engineered bacterial microcompartment shells

A Hagen, M Sutter, N Sloan, CA Kerfeld - Nature communications, 2018 - nature.com
Bacterial microcompartments (BMCs) are selectively permeable proteinaceous organelles
which encapsulate segments of metabolic pathways across bacterial phyla. They consist of …