Meiotic maturation of an oocyte is the final step of oogenesis, producing mature fertilizable
eggs. Entry of oocytes into meiosis is regulated by the input of cell signaling networks onto …

During mouse development, the gonad begins to form shortly before 10.5 days postcoitum
(dpc) on the ventromedial side of the mesonephros. The XY gonad consists of germ cells …

Publisher Summary This chapter describes a method for microinjection of oocytes, eggs,
and embryos with quantitative techniques initially used by Pierre de Fonbrune in the 1930s …

We present evidence that the embryo of the ascidian, Ciona intestinalis, is an easily
manipulated system for investigating the establishment of basic chordate tissues and …

A microneedle puncture of the fibroblast or sea urchin egg surface rapidly evokes a
localized exocytotic reaction that may be required for the rapid resealing that follows this …

The mammalian ovarian follicle consists of a multilayered complex of somatic cells that
surround the oocyte. A signal from the follicle cells keeps the oocyte cell cycle arrested at …

The ER of eggs of the sea urchin Lytechinus pictus was stained by microinjecting a
saturated solution of the fluorescent dicarbocyanine DiIC18 (3)(DiI) in soybean oil; the dye …

The mesoderm of the sea urchin embryo conventionally is divided into two populations of
cells; the primary mesenchyme cells (PMCs), which produce the larval skeleton, and the …

We use 3D time-lapse analysis of living embryos and laser scanning confocal
reconstructions of fixed, staged, whole-mounted embryos to describe three-dimensional …

Heterotrimeric kinesin-II is a plus end–directed microtubule (MT) motor protein consisting of
distinct heterodimerized motor subunits associated with an accessory subunit. To probe the …