Cell-free gene expression: an expanded repertoire of applications

AD Silverman, AS Karim, MC Jewett - Nature Reviews Genetics, 2020 - nature.com
Cell-free biology is the activation of biological processes without the use of intact living cells.
It has been used for more than 50 years across the life sciences as a foundational research …

[HTML][HTML] Wheat germ systems for cell-free protein expression

M Harbers - FEBS letters, 2014 - Elsevier
Cell-free protein expression plays an important role in biochemical research. However, only
recent developments led to new methods to rapidly synthesize preparative amounts of …

Toward microfluidic reactors for cell‐free protein synthesis at the point‐of‐care

AC Timm, PG Shankles, CM Foster, MJ Doktycz… - Small, 2016 - Wiley Online Library
Cell‐free protein synthesis (CFPS) is a powerful technology that allows for optimization of
protein production without maintenance of a living system. Integrated within micro and …

Accelerating the production of druggable targets: Eukaryotic cell-free systems come into focus

L Thoring, A Zemella, D Wüstenhagen, S Kubick - Methods and Protocols, 2019 - mdpi.com
In the biopharmaceutical pipeline, protein expression systems are of high importance not
only for the production of biotherapeutics but also for the discovery of novel drugs. The vast …

Close allies in membrane protein research: cell-free synthesis and nanotechnology

N Shadiac, Y Nagarajan, S Waters… - Molecular Membrane …, 2013 - Taylor & Francis
Membrane proteins control fundamental processes that are inherent to nearly all forms of life
such as transport of molecules, catalysis, signaling, vesicle fusion, sensing of chemical and …

Optimization of a miniaturized fluid array device for cell‐free protein synthesis

K Jackson, S Jin, ZH Fan - Biotechnology and Bioengineering, 2015 - Wiley Online Library
Cell‐free protein synthesis (CFPS), which entails synthesizing proteins outside of intact
cells, is conducted in several formats with the continuous‐exchange cell‐free (CECF) format …

Cell-free protein synthesis in microfluidic 96-well plates

K Jackson, R Khnouf, ZH Fan - Cell-Free Protein Synthesis: Methods and …, 2014 - Springer
Cell-free protein synthesis (CFPS) enables rapid protein expression for the structural and
functional characterization of proteins. Implementation of CFPS in a microfluidic platform has …

Cell-Free Synthetic Biology: The Novel Approach Towards the Biotechnological Applications

H Qaiser, M Uzair, A Arshad, JZK Khattak… - … of Synthetic Biology in …, 2023 - igi-global.com
Innovations in sustainable biomanufacturing are made possible by cell-free synthetic
biology advances. Cell-free workflows frequently combine purified enzymes with cell …