Amplification systems in immunoenzymatic techniques

S Avrameas - Journal of immunological methods, 1992 - Elsevier
Since the introduction, in 1966, of enzymes as markers for the labeling of antigens and
antibodies (Avrameas and Uriel, 1966; Nakane and Pierce, 1966), immunoenzymatic …

Electrophoretic characterization of wheat grain allergens from different cultivars involved in bakers' asthma

W Weiss, C Vogelmeier, A Görg - Electrophoresis, 1993 - Wiley Online Library
In order to identify and characterize the wheat grain allergens involved in bakers' asthma,
proteins were sequentially extracted from whole‐meal flour. The polypeptide composition of …

Simultaneous presence of different Borrelia burgdorferi genospecies in biological fluids of Lyme disease patients

I Demaerschalck, A Ben Messaoud… - Journal of clinical …, 1995 - Am Soc Microbiol
Oligonucleotide primers based on Borrelia burgdorferi sensu lato ospA gene sequences
have been designed for use in the PCR to type all (SL primers) or each (GI to GIII primers) of …

Method for enhancing fluorescence

PJ Zanzucchi - US Patent 6,118,126, 2000 - Google Patents
The present invention relates to a method for the enhancement of fluorescence wherein a
fluorophor is connected to a textured material. The method can be used in any forensic or …

Purification and characterization of microbially expressed neomycin phosphotransferase II (NPTII) protein and its equivalence to the plant expressed protein

RL Fuchs, RA Heeren, ME Gustafson, GJ Rogan… - Bio/technology, 1993 - nature.com
The gene encoding neomycin phosphotransferase II (NPTII) has been used routinely as a
selectable marker in the production of genetically engineered crops. To facilitate the safety …

Inhibition of poly (ADP-ribose) polymerase activity by Bcl-2 in association with the ribosomal protein S3a

D Song, S Sakamoto, T Taniguchi - Biochemistry, 2002 - ACS Publications
We screened a human lymphocyte cDNA library using the yeast two-hybrid system and an
automodification domain of PARP as a probe. The DNA sequence of an isolated clone …

Cysteine proteinase from the eggs of the silkmoth, Bombyx mori: identification of a latent enzyme and characterization of activation and proteolytic processing in vivo …

SY Takahashi, Y Yamamoto, Y Shionoya… - The Journal of …, 1993 - academic.oup.com
When an acid cysteine proteinase, which had been purified from the eggs of silkmoth,
Bombyx mori, was incubated at pH 3.6, enzymatic activity appeared after a few minutes, lag …

Purification and structure of rat erythroid-specific δ-aminolevulinate synthase

H Munakata, T Yamagami, T Nagai… - The Journal of …, 1993 - academic.oup.com
The existence of erythroid form δ-aminolevulinate synthase (ALAS-E) was historically a
matter of some controversy. To obtain direct evidence for a unique ALAS-E, we have purified …

Integration and expression of a rabbit liver cytochrome P-450 gene in transgenic Nicotiana tabacum.

K Saito, M Noji, S Ohmori, Y Imai… - Proceedings of the …, 1991 - National Acad Sciences
Cytochrome P-450 is involved in the oxidative metabolism of a broad range of substrates.
We have made a chimeric construct, pSN002, containing the cDNA for rabbit liver …

Cloning and expression of portions of the 34-kilodalton-protein gene of Mycobacterium paratuberculosis: its application to serological analysis of Johne's disease

M De Kesel, P Gilot, MC Misonne… - Journal of Clinical …, 1993 - Am Soc Microbiol
Paratuberculosis (Johne's disease), an endemic mycobacteriosis of cattle that is caused by
Mycobacterium paratuberculosis, is characterized by incoercible diarrhea and fecal …