Mammalian cell culture for production of recombinant proteins: A review of the critical steps in their biomanufacturing
R O'Flaherty, A Bergin, E Flampouri, LM Mota… - Biotechnology …, 2020 - Elsevier
The manufacturing of recombinant protein is traditionally undertaken in mammalian cell
culture. Today, speed, cost and safety are the primary considerations for process …
culture. Today, speed, cost and safety are the primary considerations for process …
Progress in fed-batch culture for recombinant protein production in CHO cells
WJ Xu, Y Lin, CL Mi, JY Pang, TY Wang - Applied Microbiology and …, 2023 - Springer
Nearly 80% of the approved human therapeutic antibodies are produced by Chinese
Hamster Ovary (CHO) cells. To achieve better cell growth and high-yield recombinant …
Hamster Ovary (CHO) cells. To achieve better cell growth and high-yield recombinant …
Developments and opportunities in continuous biopharmaceutical manufacturing
O Khanal, AM Lenhoff - MAbs, 2021 - Taylor & Francis
Today's biologics manufacturing practices incur high costs to the drug makers, which can
contribute to high prices for patients. Timely investment in the development and …
contribute to high prices for patients. Timely investment in the development and …
Development of an intensified fed-batch production platform with doubled titers using N-1 perfusion seed for cell culture manufacturing
The goal of cell culture process intensification is to increase volumetric productivity,
generally by increasing viable cell density (VCD), cell specific productivity or production …
generally by increasing viable cell density (VCD), cell specific productivity or production …
Biomanufacturing evolution from conventional to intensified processes for productivity improvement: a case study
Process intensification has shown great potential to increase productivity and reduce costs
in biomanufacturing. This case study describes the evolution of a manufacturing process …
in biomanufacturing. This case study describes the evolution of a manufacturing process …
Improving titer while maintaining quality of final formulated drug substance via optimization of CHO cell culture conditions in low-iron chemically defined media
During biopharmaceutical process development, it is important to improve titer to reduce
drug manufacturing costs and to deliver comparable quality attributes of therapeutic …
drug manufacturing costs and to deliver comparable quality attributes of therapeutic …
A metabolomics approach to increasing Chinese hamster ovary (CHO) cell productivity
Much progress has been made in improving the viable cell density of bioreactor cultures in
monoclonal antibody production from Chinese hamster ovary (CHO) cells; however, specific …
monoclonal antibody production from Chinese hamster ovary (CHO) cells; however, specific …
Identification of novel inhibitory metabolites and impact verification on growth and protein synthesis in mammalian cells
Mammalian cells consume large amount of nutrients during growth and production.
However, endogenous metabolic inefficiencies often prevent cells to fully utilize nutrients to …
However, endogenous metabolic inefficiencies often prevent cells to fully utilize nutrients to …
Quantifying the impact of cell culture media on CHO cell growth and protein production
M Combe, S Sokolenko - Biotechnology Advances, 2021 - Elsevier
In recombinant protein production, cell culture media development and optimization is
typically seen as a useful strategy to increase titer and cell density, reduce by-products, as …
typically seen as a useful strategy to increase titer and cell density, reduce by-products, as …
Enhancing early-stage cell culture process development efficiency using an integrated approach of high-throughput miniaturized bioreactors and definitive screening …
Rapidly expanding pipelines in biopharmaceutical industry and increasingly stringent
requirements from regulatory agencies call for a more efficient and rational study design at …
requirements from regulatory agencies call for a more efficient and rational study design at …