Here, we review the current state of the art in analytical and structural methods used to
characterize therapeutic monoclonal antibodies (mAbs) and derivatives. First, a general …

Introduction: Hydrogen deuterium exchange mass spectrometry (HDX-MS) is a powerful
methodology to study protein dynamics, protein folding, protein-protein interactions, and …

Highlights•Hydrogen/deuterium exchange mass spectrometry (HDX-MS) technology for
assessment of higher order structure of protein therapeutics.•Methodology: global HDX-MS …

Assessing the functional outcome of protein interactions in structural terms is a goal of
structural biology, however most techniques have a limited capacity for making structure …

Fc galactosylation is a critical quality attribute for anti-tumor recombinant immunoglobulin G
(IgG)-based monoclonal antibody (mAb) therapeutics with complement-dependent …

Food allergy is the disease where the immune system is elicited by antigens in food.
Although innocuous for immune-tolerant individuals, an ever-growing number of food …

With the increased global awareness and rise in food allergies, a multifold interest in food
allergens is evident. The presence of undeclared food allergens results in expensive food …

Mass spectrometric epitope mapping has become a versatile method to precisely determine
a soluble antigen's partial structure that directly interacts with an antibody in solution. Typical …

Protein therapeutics have emerged as a major class of biopharmaceuticals over the past
several decades, a trend that has motivated the advancement of bioanalytical technologies …

Food allergy is receiving increased attention in recent years. Because there is currently no
known cure for food allergy, avoiding the offending food is the best defense for sensitive …