Gene editing stands for the methods to precisely make changes to a specific nucleic acid
sequence. With the recent development of the clustered regularly interspaced short …

Climate change gives rise to numerous environmental stresses, including soil salinity.
Salinity/salt stress is the second biggest abiotic factor affecting agricultural productivity …

Based on engineered or bacterial nucleases, the development of genome editing
technologies has opened up the possibility of directly targeting and modifying genomic …

Gene editing that makes target gene modification in the genome by deletion or addition has
revolutionized the era of biomedicine. Clustered regularly interspaced short palindromic …

Long-range gene editing by homology-directed repair (HDR) in hematopoietic
stem/progenitor cells (HSPCs) often relies on viral transduction with recombinant adeno …

Gene therapy has long held promise to correct a variety of human diseases and defects.
Discovery of the Clustered Regularly-Interspaced Short Palindromic Repeats (CRISPR), the …

CRISPR–Cas9 nucleases are widely used for genome editing but can induce unwanted off-
target mutations. Existing strategies for reducing genome-wide off-target effects of the widely …

With the recent development of CRISPR technology, it is becoming increasingly easy to
engineer the genome. Genome-editing systems based on CRISPR, as well as transcription …

Recent clinical trials of gene therapy have shown remarkable therapeutic benefits and an
excellent safety record. They provide evidence for the long-sought promise of gene therapy …

CRISPR RNA-guided nucleases (RGNs) are widely used genome-editing reagents, but
methods to delineate their genome-wide, off-target cleavage activities have been lacking …