Recent advances in cryo-electron microscopy have marked only the beginning of the
potential of this technique. To bring structure into cell biology, the modality of cryo-electron …

In a rapidly growing yeast cell, 60% of total transcription is devoted to ribosomal RNA, and
50% of RNA polymerase II transcription and 90% of mRNA splicing are devoted to ribosomal …

Passive macromolecular diffusion through nuclear pore complexes (NPCs) is thought to
decrease dramatically beyond a 30–60-kD size threshold. Using thousands of independent …

Fluorescence light microscopy allows multicolor visualization of cellular components with
high specificity, but its utility has until recently been constrained by the intrinsic limit of spatial …

The nucleus is one of the most prominent cellular organelles, yet surprisingly little is known
about how it is formed, what determines its shape and what defines its size. As the nuclear …

The nuclear envelope (NE) is a highly regulated membrane barrier that separates the
nucleus from the cytoplasm in eukaryotic cells. It contains a large number of different …

The functional state of a cell is largely determined by the spatiotemporal organization of its
proteome. Technologies exist for measuring particular aspects of protein turnover and …

It is not known how the volume of the cell nucleus is set, nor how the ratio of nuclear volume
to cell volume (N/C) is determined. Here, we have measured the size of the nucleus in …

We investigate the relationship of protein-protein interactions with mRNA expression levels,
by integrating a variety of data sources for yeast. We focus on known protein complexes that …

A long-standing biological question is how a eukaryotic cell controls the size of its nucleus.
We report here that in fission yeast, nuclear size is proportional to cell size over a 35-fold …