In-cell structural biology aims at extracting structural information about proteins or nucleic
acids in their native, cellular environment. This emerging field holds great promise and is …

A detailed knowledge of the complex processes that make cells and organisms alive is
fundamental in order to understand diseases and to develop novel drugs and therapeutic …

NMR spectroscopy has been applied to cells and tissues analysis since its beginnings, as
early as 1950. We have attempted to gather here in a didactic fashion the broad diversity of …

Classical structural biology approaches allow structural characterization of biological
macromolecules in vitro, far from their physiological context. Nowadays, thanks to the wealth …

Studying the structural aspects of proteins within sub-cellular compartments is of growing
interest. Dynamic nuclear polarization supported solid-state NMR (DNP-ssNMR) is uniquely …

In-cell NMR spectroscopy is a unique tool for characterizing biological macromolecules in
their physiological environment at atomic resolution. Recent progress in NMR instruments …

Conspectus Cellular structural biology methods are needed to characterize biological
processes at atomic resolution in the physiological environment of the cell. Toward this goal …

Cytochrome c (cyt c) is a multifunctional protein with varying conformations. However, the
conformation of cyt c in its native environment, mitochondria, is still unclear. Here, we …

Conventional structural and chemical biology approaches are applied to macromolecules
extrapolated from their native context. When this is done, important structural and functional …

In-cell nuclear magnetic resonance (NMR) is a method to provide the structural information
of a target at an atomic level under physiological conditions and a full view of the …